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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
TCR vβ8.3
- 级别:
TCR vβ8.3 Antibody
- 目录编号:
TCR vβ8.3 Antibody
- 克隆性:
单克隆
- 抗原来源:
仓鼠
- 保质期:
TCR vβ8.3 Antibody
- 抗体英文名:
anti-mouse TCR vβ8.3 Antibody-PE
- 抗体名:
TCR vβ8.3 Antibody
- 标记物:
PE
- 宿主:
仓鼠
- 适应物种:
小鼠
- 免疫原:
TCR vβ8.3 Antibody
- 亚型:
TCR vβ8.3 Antibody
- 形态:
TCR vβ8.3 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2100.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥4400.0 |
Description :The TCR Vβ8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. 1B3.3 reacts withVβ8.3 only, but not Vβ8.1 or Vβ8.2. Most mouse strains have about 20% Vβ8+ T cells in theperiphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrchaplotype) do not express Vβ8 due to a gene deletion at the Vβ8 loci. Exogenoussuperantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ8 andselectively eliminate those T cells in vivo.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :3.L2 T Cell Clone Expressing the vβ8.3 TCR
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per millioncells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance foreach application.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Kersh G, et al. 1998. J. Immunol. 161:585.
Product Citations:
1. Fu X, et al. 2020. J Immunol. 205:2066. PubMed
2. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
3. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
,T 和 B 细胞在免疫系统中展现两种不同功能的识别机制。T 细胞识别细胞膜表面 MHC I 或 II 类分子呈递的抗原肽来识别细胞。而 B 细胞没有选择识别机制。近几年,TCR 样抗体研发工作取得较大进展,许多研究团队都具备制作该抗体能力,研发出了许多针对肿瘤和病毒 T 细胞表位的 TCR 样抗体。能够获得被 T 细胞识别的,纯化后自然折叠 MHC-短肽复合物,是 TCR 样抗体制备关键。MHC-短肽复合物可以通过大肠杆菌表达获得。大肠杆菌分别表达 MHC 重链和β-球蛋白,在体外折叠后,与短肽
条带。 五、 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 六、双重染色抗体的选择 用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 七、荧光和化学发光标记 连接于二抗的标记











