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- 2025年07月13日
- ELISA Detection
- 小鼠
- 人、小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
TGF-β1
- 级别：
TGF-β1 Antibody
- 目录编号：
TGF-β1 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
TGF-β1 Antibody
- 抗体英文名：
Biotin anti-human/mouse TGF-β1 Antibody
- 抗体名：
TGF-β1 Antibody
- 标记物：
TGF-β1 Antibody
- 宿主：
小鼠
- 适应物种：
人、小鼠
- 免疫原：
TGF-β1 Antibody
- 亚型：
TGF-β1 Antibody
- 形态：
TGF-β1 Antibody
- 应用范围：
ELISA Detection
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
50 µg
Description ：TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes.TGF-β1 is synthesized as a 390 amino acid precursor that is cleaved by furin, localized in thetrans-Golgi network, in residue 278. Furin processes the TGF-β1 precursor at the carboxylside of the consensus sequence RHRR which precedes the NH2-terminal Ala 279 residue ofthe mature TGF-β1. The TGF-β1 precursor includes the latency-associated peptide (LAPdimer) in the N-terminal portion and the 25 kD portion that constitutes the mature TGF-β1 inthe C-terminal. LAP dimer and the TGF-β1 mature protein remain non-covalently associatedafter furin cleavage and this complex does not bind to the TFG-β1 receptor. In addition, theTGF-β1 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active formrequires dissociation from LAP. Some activators can release TGF-β1 from LAP such asthrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8.
Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity.Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be anessential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of humanTh17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17differentiation in a dose-dependent manner. While TGF-β1 is required for the expression ofRORgt, in human naïve CD4+ T cells from cord blood, TGF-β1 can inhibit the function ofRORgt at high doses. By using serum-free medium, it has been clarified that the optimumconditions for human Th17 differentiation are TGF-β1, IL-1b, and IL-2 in combination with IL-6,IL-21, or IL-23.
Verified Reactivity ：Human, Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Human TGF-β1
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：ELISA Detection - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.0625 µg/mlof the detection antibody was utilized to generate the example standard curve. It is recommendedthat the end-user titrate each lot of reagents for optimal performance.
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文献和实验1. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
2. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
野生型p53蛋白可以通过诱导凋亡而抑制肿瘤的生长，突变型p53基因丧失了诱导细胞凋亡的能力，从而增强了细胞的恶性转化能力，而且在恶性肿瘤常可检测出突变型p53基因。p53蛋白也可能参与化疗药物诱导的细胞凋亡。本法运用一步夹心免疫法，定量检测野生型或突变型p53蛋白。 样本来源 1. 人、鼠、兔的组织切片 2. 肿瘤细胞株 材料与试剂 1. 过氧化物酶标记的多克隆抗人-p53全抗体（检测抗体）； 2. 洗涤缓冲液（10x）； 3. 过氧化物酶底物液： 四甲基联苯
胶体金的pH值时可用0.1molK2CO3，需要降低胶体金的pH值时可用0.1N HCl.测定金溶液的pH可能损害pH测定计的探头，因此，一般用精密的pH试纸测定其pH即可。几种常用的蛋白质标记时胶体金所用的pH值见表5-4.表5-4 常用几种蛋白质标记时胶体金所用的pH值如下 蛋白质pH抗体（γ球蛋白）9.0亲合层析的IgG7.6单克隆抗体8.2F（ab＇）27.2SPA(葡萄球菌蛋白)6.0蓖麻子植物凝血素Ⅰ8.0蓖麻子植物凝血素Ⅱ8.0花生凝集素6.3Helix pomatia lectin
