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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
TotalSeq™-A0182 anti-mouse CD3 Antibody
- 抗体名:
CD3 Antibody
- 标记物:
CD3 Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
PG
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
10 ug
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
2. Golomb SM, et al. 2020. Cell Rep. 33:108438. PubMed
3. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
4. Guldner IH, et al. 2021. STAR Protocols. 2(2):100537. PubMed
5. Pisu D, et al. 2021. J Exp Med. 218:. PubMed
- 3) 及 CD45 -PE (HI30,白细胞标志) 抗体染色后,进行流式分析,死细胞以 DAPI 染色先行排除,左右分别为分选前后的细胞群分布。 总结: 几次实验的结果,使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选,平均得率为 84.5%,纯度大于 90%,细胞存活率高于 95%,且目标细胞不含标记,贴近天然,使下游实验更精确。
大学的Andrew Chalmers博士于2014年主导创立的,其号称是世界最大的抗体搜索引擎,该网站上包含了来自204个抗体供应商的近五百万个抗体,汇总了两百多万条抗体引用数据,并且该网站仍在不断更新最新的数据。输入关键词(如:CD3)后点击搜索即可出现所有符合条件的抗体,而该网站的特色是,搜索结果根据抗体被文献引用的次数排序,一般来说,引用越多的产品,质量往往越有保证。 03 Labome 网址:https://www.labome.com/ Labome(www.labome.com
及释放目标细胞。以不具磁性且可逆的试剂,取代一般具高亲和力的完整抗体,从而达到直接由全血或其他样本,进行温和标准化正选的目的。Fab-TACS 正选,化解了传统使用完整抗体进行正选的困境,其对目标细胞无不必要的刺激,避免了分选流程的细胞活化,也避免完整抗体与特定受体的结合而影响的分析准确度。因此,使用 Fab-TACS 无痕正选技术可富集不含标记、未激活的目标细胞,次次精准。人全血 CD3+ T 细胞分选实例1. 所需产品:(1) CD3 Fab-TACS® Gravity Kit, human











