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- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Nanog
- 级别:
Nanog Antibody
- 目录编号:
Nanog Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
Nanog Antibody
- 抗体英文名:
Alexa Fluor® 488 anti-Nanog Antibody
- 抗体名:
Nanog Antibody
- 标记物:
Nanog Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
Nanog Antibody
- 亚型:
Nanog Antibody
- 形态:
Nanog Antibody
- 应用范围:
ICC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 ug
Description :Nanog is a homeodomain-containing transcription factor that is essential for early embryonicdevelopment. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer celllines, maintaining the undifferentiated status of the cells and is downregulated upondifferentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineagedifferentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 andSOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog isregulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in severaltypes of tumor cells. The expression level of Nanog in cancer cells has been shown to becorrelated with the malignancy of cancers.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Full length NANOG recombinant protein expressed in E. coli.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunocytochemistry. Forimmunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. It isrecommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Excitation Laser :Blue Laser (488 nm)
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CST 抗体 9 月特惠|购买任意 CST 抗体,+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
抗G4S linker抗体:一文讲清"通用连接肽"检测的原理与选型
比例。 04 抗G4S linker抗体使用注意事项 识别的是重复表位:多数抗G4S linker单抗针对(G4S)n重复,n=3及以上信号最强,单段G4S需先做预实验; 与Whitlow linker不交叉:序列完全不同,需用对应的抗Whitlow抗体,不要混用; 必须设阴性对照:用未转导的同谱系细胞(如Jurkat)设阴性门,否则易把背景染成阳性; 偶联物选择:多色流式优先选直接荧光偶联(Alexa Fluor 647、PE等),减少二抗串扰; 临床转化阶段:进入GMP或IND相关检测时,优先选
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