- ￥6500
- 齐源
- 674204
- 国产/进口
- 2025年07月15日
- ICC, IHC-P
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Nanog
- 级别：
Nanog Antibody
- 目录编号：
Nanog Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
Nanog Antibody
- 抗体英文名：
Alexa Fluor® 594 anti-Nanog Antibody
- 抗体名：
Nanog Antibody
- 标记物：
Nanog Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
Nanog Antibody
- 亚型：
Nanog Antibody
- 形态：
Nanog Antibody
- 应用范围：
ICC, IHC-P
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
100 ug
Description ：Nanog is a homeodomain-containing transcription factor that is essential for early embryonicdevelopment. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer celllines, maintaining the undifferentiated status of the cells and is downregulated upondifferentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineagedifferentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 andSOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog isregulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in severaltypes of tumor cells. The expression level of Nanog in cancer cells has been shown to becorrelated with the malignancy of cancers.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Full length NANOG recombinant protein expressed in E. coli.
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594under optimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICC - Quality tested、IHC-P - Verified
Recommended Usage ：Each lot of this antibody is quality control tested by immunocytochemistry. Forimmunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. Forimmunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentrationrange of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimalperformance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
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文献和实验保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF543 标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。CF 555、AlexaFluor 555、Tetramethylrhodamine(TAMRA) 等匹配Cy3滤光片的橙色荧光染料；红色（568-594nm处激发）CF 568、Alexa Fluor® 568,ATTO 565, Rhodamine Red等568nm处红色荧光染料；CF 568染料耐光性最好；高效水溶
，耐光，水溶性好，确保了CF 543染料与抗体的耦联物保持优异的水溶性，为该波段最亮的橙色荧光染料。例如：同等的标记程度下，CF 543标记的羊抗鼠IgG抗体的亮度高于用Alexa Fluor 546 标记的2~10倍。 CF 555、Alexa Fluor 555、Tetramethylrhodamine (TAMRA) 等匹配Cy3滤光片的橙色荧光染料。 4、红色（568-594nm处激发） CF 568、Alexa Fluor® 568, ATTO 565
技术原理与方法｜超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色（Alexa Fluor405，蓝色）；CB1 染色（Alexa Fluor488，绿色）；VGluT3 染色（Cy3，红色）；DGLα染色（Alexa Fluor647，白色）。 大麻素合成酶 DGLα（白色）和大麻素受体 CB1（绿色）聚集在小鼠
