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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
ZAP70 Phospho
- 级别：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 目录编号：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 抗体英文名：
anti-ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody-PE
- 抗体名：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 亚型：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 形态：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥5900.0 |
Description ：ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinasethat is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and therelated spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation.This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR)stimulation, functions in the initial step of TCR-mediated signal transduction in combination withthe Src family kinases, Lck and Fyn.
ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and byphosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr-315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr-292has a negative regulatory role.
Phosphorylated Y319 is a positive regulator of ZAP70, which triggers the binding of Lck andactivation of NFAT and IL-2 induction. The binding of Lck to phosphorylated Y319 promotesLck mediated phosphorylation of Y493, and facilitates activation of downstream signaling. Theimportance of Y319 phosphorylation in positive regulation of ZAP70 was confirmed in a mousemodel with Y319F ZAP70 mutant expression, which resulted in severe defects in calciummobilization and thymocyte selection.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Modified peptide
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. He X, et al. 2018. EMBO J. 37:e97980. PubMed
有了质量好的抗原，还必须选择适当的免疫途径，才能产生质量好（特异性强和效价高）的抗体。 （一）用于免疫的动物 作免疫用的动物有哺乳类和禽类，主要为羊、马、家兔、猴、猪、豚鼠、鸡等，实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量，如一般制备抗r-免疫球蛋白抗血清，多用家兔和山羊，因动物反应良好，而且能够提供足够数量的血清，用于免疫的动物应适龄
面，抗体的结合可以增强免疫细胞对抗原的识别和吞噬能力，进而增加免疫应答。另一方面，抗体的结合可以通过与免疫细胞表面的调节受体结合，抑制或增强免疫应答反应，帮助免疫系统在抗感染，抗肿瘤等过程中取得平衡的应答反应。 4、 抗原抗体复合物通过经典途径激活补体系统 补体系统是一种能够引起炎症和细胞溶解的蛋白质系统，IgG1、IgG2、IgG3和IgM类抗体与抗原结合形成免疫复合物激活补体系统，从而发挥补体介导的杀菌、溶菌作用。清除细菌、病毒等病原体。这主要在抗细菌和抗寄生虫感染中起作用。 5、介导细胞
，对某些传染病如甲型肝炎有较高的临床诊断价值。 抗原抗体反应 1. 可逆性 抗原与抗体结合形成抗原抗体复合物的过程是一种动态平衡，其反应式为： Ag+Ab→Ag·Ab 抗体的亲和力（affinity）是抗原抗体间的固有结合力，可以用平衡常数K表示： K=[Ag·Ab]／[Ag][Ab] Ag·Ab 的解离程度与K值有关。高亲和力抗体的抗原结合点与抗原的决定簇在空间构型上非常适合，两者结合牢固，不易解离。解离后的抗原或抗体均能保持原有的结构和活性，因此可用亲和层析法来提纯抗原或抗体。在抗
