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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
9999
- 靶点：
CD45.2
- 级别：
anti-mouse CD45.2 Antibody
- 目录编号：
anti-mouse CD45.2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-mouse CD45.2 Antibody
- 抗体英文名：
Alexa Fluor® 488 anti-mouse CD45.2 Antibody
- 抗体名：
CD45.2 抗体
- 标记物：
anti-mouse CD45.2 Antibody
- 宿主：
小鼠
- 适应物种：
小鼠
- 免疫原：
anti-mouse CD45.2 Antibody
- 亚型：
anti-mouse CD45.2 Antibody
- 形态：
anti-mouse CD45.2 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
25 ug/100 ug
| 规格： | 25 ug | 产品价格： | ￥1700.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥3900.0 |
Description ：CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：B10.S mouse thymocytes and splenocytes
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488under optimal conditions
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser ：Blue Laser (488 nm)
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文献和实验1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
2. Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
3. Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed
4. Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed
5. Wen T, et al. 2013. Proc Natl Acad Sci U S A. 110:6067. PubMed
6. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
7. Wang L, et al. 2020. Cells. 0.458333333. PubMed
8. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
9. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
10. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
11. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
12. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
偶联物，最大限度的提高了信噪比。图2. CF染料的超强特异性；由于AF染料制备过程中含有大量带负电的磺化基团，容易非特异性结合正电基团，请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解，会在运输，操作及储存中带来诸多不便，最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性，恶化水解作用等问题。例如： AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
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Fluor® 488 and PE channels Eliminate or correct for cell aggregates during data analysis using gating or modeling software Human and rodent stem cells efflux Vybrant® DyeCycle™ Violet stain, and this is the basis for the Side Population
