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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
TSLPR (TSLP-R)
- 级别:
TSLPR (TSLP-R) Antibody
- 目录编号:
TSLPR (TSLP-R) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
TSLPR (TSLP-R) Antibody
- 抗体英文名:
anti-human TSLPR (TSLP-R) Antibody-PE
- 抗体名:
TSLPR (TSLP-R) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
TSLPR (TSLP-R) Antibody
- 亚型:
TSLPR (TSLP-R) Antibody
- 形态:
TSLPR (TSLP-R) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
TSLPR (TSLP-R) Antibody
- 规格:
100 tests
Description :TSLP-R, also known as thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2,CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complexwith IL-7R to bind TSLP. The TSLP-R contains a WSXWS motif required for proper proteinfolding and a box1 motif important for association with the JAKs. TSLP-R has a predictedmolecular weight approximately 40 kD, and two isoforms have been reported that are producedby alternative splicing. The TSLP-R is expressed preferentially in myeloid cells includingdendritic cells and activated monocytes, and is weakly expressed in T cells. Expression hasalso been reported in heart, skeletal muscle, and kidney tissues. TSLP binding to theheterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3 and STAT5 tostimulate cell proliferation. Ligand receptor interactions haves been implicated in thedevelopment of the hematopoietic system, dendritic cell maturation, and the maintenance andpolarization of human Th2 memory T cells in allergic diseases.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human TSLP-R:Fc protein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentrationand Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Bouchlaka MN, et al. 2013. J Exp Med. 210:2223. PubMed
Product Citations:
1. Nováková M, et al. 2020. Haematologica. . PubMed
2. Bouchlaka M, et al. 2013. J Exp Med. 210:2223. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
以「出汗」的方式排出脂肪!Science 揭示这种细胞因子或
细胞)没有出现明显的 WAT 减少。因此,αβ T 细胞是 TSLP 驱动的脂肪减少所必需的。为了区分是 CD4+ 还是 CD8+ T 细胞导致了这种效应,他们使用抗体清除 CD4+和/或 CD8+ T 细胞。出乎意料的是,只有消除所有类型 T 细胞才能防止 TSLP 介导的 WAT 损失,说明所有 T 细胞亚群都是所必需的。图片来源:ScienceTSLP 通过增加皮脂分泌来诱导脂肪流失TSLP 在小鼠体内造成了脂肪的减少,因此热量输出大于热量摄入。然而,热量摄入的减少并不是 TSLP 诱导的脂肪损失
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗,这种抗体有单抗的吧? 用于FCM: 1:20-100,这是说用于流式的稀释倍数,是不是稀释倍数越小越好呢? 同型对照抗体是不是必须要做的?如何选择阴型同型对照抗体呢! 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助! freecell 抗体的稀释倍数是需要优化











