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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD64
- 级别:
anti-human CD64 Antibody-FITC
- 目录编号:
anti-human CD64 Antibody-FITC
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human CD64 Antibody-FITC
- 抗体英文名:
anti-human CD64 Antibody-FITC
- 抗体名:
CD64抗体
- 标记物:
FITC
- 宿主:
小鼠
- 适应物种:
人、猴
- 免疫原:
anti-human CD64 Antibody-FITC
- 亚型:
anti-human CD64 Antibody-FITC
- 形态:
anti-human CD64 Antibody-FITC
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
anti-human CD64 Antibody-FITC
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥3200.0 |
Description :CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is amember of the immunoglobulin superfamily and is expressed on monocytes/macrophages,dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γstimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosisof IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Verified Reactivity :Human, Cynomolgus, Rhesus
Reported Reactivity :Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Blue Laser (488 nm)
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文献和实验1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. Holl V, et al. 2004. J. Immunol. 173:6274.
5. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6. Cho HJ, et al. 2007. Physiol Genomics 149:60.
7. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8. Bruhns P, et al. 2008. Blood 113:3716. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Carter DL, et al. 1999. Cytometry 37:41. (FC)
11. Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Product Citations:
1. Oostindie SC, et al. 2022. Nat Biotechnol. :. PubMed
2. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
3. Lara S, et al. 2021. Eur J Immunol. . PubMed
4. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
5. Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成; 方法与步骤: 根据Marshall氏法高效价的抗人球蛋白兔免疫血清,分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg,缓冲液为总量的10%; 2. 将以上溶液降温至4℃,按蛋白:荧光素=50—80mg:1mg的比例加入异硫氰酸荧光素,在0—4℃下电磁搅拌12—14h; 3.用半饱和硫酸铵将标记球蛋白
#01 什么是同型对照? 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照? 流式抗体与靶标的结合,是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞(除T细胞外)表面大多表达 CD16、CD32、CD64 等 Fc 受体,这些受体可与各种抗体的 Fc 端非特异性结合,出现非特异










