- 询价
- BIO-RAD
- MCA756F
- 2025年10月17日
- F, IF
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human monocytes
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
10.1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IF
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD64 antibody | 10.1
- 规格：
0.1 mg
Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI.
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
| Immunofluorescence | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
Source Reference
- Dougherty, G.J. et al. (1987) The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.
Eur J Immunol. 17 (10): 1453-9.
References for CD64 antibody
- Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73. - Fadlon,. E. et al. (1998) Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64.
Blood. 91: 266-74. - Navarro-López, F. et al. (2003) Late T-lymphocyte and monocyte activation in coronary restenosis. Evidence for a persistent inflammatory/immune mechanism?
Rev Esp Cardiol. 56: 465-72. - Beekman, J.M. et al. (2004) Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.
Proc Natl Acad Sci U S A.101: 10392-7. - Kahn, F. et al. (2008) Antibodies against a surface protein of
Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149. - Wagner, C. et al. (2008) T lymphocytes in acute bacterial infection: increased prevalence of CD11b(+) cells in the peripheral blood and recruitment to the infected site.
Immunology. 125: 503-9. - Eisenhardt, S.U. et al. (2009) Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.
Circ Res. 105: 128-37. - Tanaka, M. et al. (2009) Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.
J Immunol. 183: 2349-55.
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文献支持
Mouse anti Human CD64:FITC，clone 10.1
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