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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD223
- 级别:
CD223 (LAG-3) Antibody
- 目录编号:
CD223 (LAG-3) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD223 (LAG-3) Antibody
- 抗体英文名:
Biotin anti-human CD223 (LAG-3) Antibody
- 抗体名:
CD223 (LAG-3) 抗体
- 标记物:
CD223 (LAG-3) Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD223 (LAG-3) Antibody
- 亚型:
CD223 (LAG-3) Antibody
- 形态:
CD223 (LAG-3) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 µg
Description :CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved inT-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells(NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated Tcells in acute conditions but high expression is maintained under tolerizing conditions. CD223deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverseexhausted phenotypes, improve tumor rejection, and control viral load.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human LAG-3 transfected cells.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with biotin under optimalconditions.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验生物素亲和素(又称抗生物素)系统(biotin avidin system,BAS)标记抗体的技术是 20 世纪 70 年代后期发展起来的一种新的免疫检测方法。 由于亲和素与生物素间的亲和力极强,结合迅速,且极其稳定,生物素标记抗体和酶的标记率高,又不影响蛋白的活性,使 BAS 标记技术比常规酶联免疫、放射免疫及荧光免疫技术有着更高的灵敏度,为微量抗原、抗体的检测开辟了新的途径。近年来,在 BAS 检测中多用链霉亲和素“streptavidin,SA”,它是链霉菌培养过程中的分泌物,完整的链霉
本法可使抗体或其它蛋白质的ε-氨基通过手臂与酰化的生物素共价结合。其后,生物素化的分子可应用酶标-亲和素或荧光染料-链霉亲和素复合物来检测。小分子的水溶性生物素对细菌蛋白链霉亲和素具有高度亲和力是本法的设计基础。 NHSB:N-羟基琥珀酰亚胺生物素(有商品供应) 0.1 mol/L 碳酸氢钠缓冲液,pH 8.0 双蒸水(注意去除游离氨基),用以配制碳酸氢钠缓冲液或硼酸缓冲液 DMSO(二甲亚砜,有毒试剂) 1 mol/L NH4Cl 叠氮钠(有毒试剂) PBS
点。给动物饲以大量鸡蛋白所致的维生素H缺乏,就是由于卵白素和大量的维生素H结合所致。维生素H(以下统称生物素)是一种小分子的维生素,分子量为244,系转氨甲酰基化过程中的辅酶,它与卵白素之间有很强的亲合力,较之抗体对抗原的亲合力要高出100万倍,能够彼此牢固结合而不影响彼此的生物学活性。同时,生物素与卵白素都具有与其它示踪物质如荧光素、铁蛋白和过氧化酶等相结合的能力。免疫细胞化学工作者利用上述特性,建立了卵白素―生物素免疫染色系统。由于维生素H习惯上称为生物素,为便于理解,现在译名上统称卵白素为抗生物素










