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文献和实验Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed
SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
. Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum® Taq DNA Polymerase High Fidelity is an enzyme mixture composed of recombinant Taq DNA polymerase
Expand High Fidelity PCR--高保真PCR
Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products
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