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文献和实验SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
实验原理 The system consists of two major components: SuperScript™ III RT/ Platinum® Taq High Fidelity Enzyme Mix and 2X Reaction Mix. SuperScript™ III Reverse Transcriptase is a version of M-MLV RT that has been engineered
Expand High Fidelity PCR--高保真PCR
will have at least one mutation. See Boehringer data sheet for more details on error rate. Mix in an 0.2 ml thin wall microfuge tube: 1 microliter each: dATP, dGTP, dCTP, dTTP (10 mM solutions) 100 ng each primer (20-mer) 5 microliters Expand buffer +15 mM MgCl2
Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
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