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文献和实验Expand High Fidelity PCR--高保真PCR
Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products
The Polymerase Chain Reaction (PCR)
and multi-block systems. •Reactions in tubes or 96-well micro-titre plates. So, I Can Just Go Ahead? -Not so fast. -The PCR technique and the use of Taq DNA polymerase in PCR are both patented. -Even academic and public organisations must pay
A fast, simple, and reliable high-yielding method for DNA extraction from different plant species
Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species
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