Hot Start Core Kit Apta+ 试剂盒，o

FFPE Direct PCR Kit

°C for X min(1min/kb)        Final extension 72°C for 7 min        Final soak 4-10°C    6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling

Plant Seed Direct PCR Kit

  6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling program.    7) Simplified hot start: Start the PCR program. Once the thermal cycler has reached 94°C

Protocol for Real-Time RT-PCR

and melting curve analysis, give misleading real-time PCR result. To avoid this problem, please make sure to perform hot-start PCR and use at least 60°C annealing temperature. We noticed not all hot-start Taq polymerases are equally efficient at suppressing