- ￥27393.25
- Enzo Life Sciences
- ENZ-GEN440-0020
- 美国
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
欣博盛生物
- 应用：
Fluorescent detection
- 规格：
20 tests
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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
are independent from the target sequence. As a result, practically any SNP assay can use primary probes designed with different target-specific regions, but the identical two 5' flap sequences. . 3. The design of the invasive probe starts with its 3' terminal
transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA
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