产品说明: Clone 3E8 (See other available formats) Regulatory Status RUO Other Names High mobility group protein 1, High mobility group protein B1, Sulfoglucuronyl carbohydrate binding protein Isotype Mouse IgG2b, κ
描述 高迁移率组蛋白B1(HMGB1)属于一个高度保守的蛋白质家族,包含HMG-box结构域。人HMGB1表达为215个氨基酸(aa)的单链多肽,包含三个结构域:两个N末端球状、70个带正电荷的DNA结合结构域(HMG框a和B)和一个带负电荷的30个氨基酸的C末端区,仅包含Asp和Glu。人HMGB1与犬HMGB1具有100%aa的相同性,与小鼠、大鼠、牛和猪HMGB1的相同性为99%。 HMGB1是一种广泛表达且高度丰富的蛋白质。它最初被发现是一种可以弯曲DNA的核蛋白。这种弯曲稳定核小体的形成,并在DNA结合蛋白募集时调节选择基因的表达。目前已知HMGB1在与炎症相关的细胞外信号传导中也起着重要作用。HMGB1在细胞坏死过程中大量释放到细胞外环境中。它是一种促进单核细胞迁移和细胞因子分泌的炎症介质,也是T细胞-树突状细胞相互作用的介质。此外,活化的单核细胞、巨噬细胞和树突状细胞也分泌HMGB1,形成正反馈回路,导致释放额外的细胞因子和中性粒细胞。HMGB1的细胞因子活性仅限于HMG B盒,而A盒与转录因子的螺旋-环-螺旋结构域相关。尽管HMGB1不具有经典的信号序列,但它似乎是通过分泌性内溶酶体胞吐作用以乙酰化形式分泌的。一旦分泌,HMGB1通过其高亲和力受体RAGE以及可能的TLR2和TLR4转导细胞信号。 High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1.
HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.
Product Details Verified Reactivity Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Immunogen Recombinant human HMGB1 with GST tag. A universal T cell epitope from a Mycobacterium tuberculosis antigen was introduced into the C-terminus of HMGB1 to increase the immunogenicity.
Preparation The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Antigen Details Structure 215 amino acids with predicted molecular weight of 25 kD. Distribution Nucleus
Function DNA binding protein that associates with chromatin and has the ability to bend DNA. Binds single-stranded DNA preferentially. Involved in V(D)J recombination by acting as a cofactor of the RAG complex.
Interaction Component of the RAG complex composed of core components RAG1 and RAG2. Cell Type B cells Biology Area Cell Biology, Immunology, Transcription Factors Molecular Family Nuclear Markers
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