Preparation:The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration:Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:ICFC - Quality tested
Recommended Usage:Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser:Red Laser (633 nm)
Application Notes:Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.
Application References: 1.Herling M, et al. 2008. Blood 111:328 2.Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123