产品简介:
BioDynami开发了NGS cfDNA建库试剂盒(illumina和MGI平台),以1 ng到50 ng的细胞游离DNA作为输入,用于构建高质量的细胞游离DNA文库。该试剂盒具有简单的工作流程和快速的程序。根据索引类型,细胞游离DNA文库的多路复用是可能的。
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
NGS cfDNA建库试剂盒工作流程
游离DNA (cfDNA)的主要来源来源于血液中凋亡的造血细胞,并在血浆中发现。cfDNA的长度约为150-200 bp。来源于恶性肿瘤的循环肿瘤DNA (ctDNA)是cfDNA的一部分。cfDNA和ctDNA均可作为无创生物标志物,因为与有创组织活检相比,cfDNA提供了更好的方法。
NGS可提供全基因组水平的分子谱分析,因此已被用于液体活检领域的cfDNA和ctDNA测序。cfDNA测序的障碍之一是难以从血浆中获得数量有限的cfDNA制备文库。BioDynami的NGS cfDNA建库试剂盒可以在1.5小时内从有限的输入中轻松获得足够的文库。
The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.
NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.
illumina平台的NGS细胞游离DNA库准备试剂盒有三种index类型:
Non-index(Cat.# 30029):没有索引。
Index(Cat.# 30031):每个索引引物包含一个独特的6个碱基索引序列,可以用于样本识别。总共48个库复用是可能的。
Unique dual index(Cat.# 30033):使用独特的双指数,可实现多达96个样本的无细胞DNA库复用。我们建立了一个Four-Base Difference Index System。该系统在8个碱基索引长度中至少有4个彼此不同的碱基。引物有效地减少了测序错误,如错误赋值、指数跳跃、指数污染等。
MGI平台试剂盒(Cat.# 34031)也适用上述index分类。
Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:
Non-index (Cat.# 30029): Libraries do not have index.
Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48 library multiplexing is possible.
Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc.
Indexes are available for the MGI platform kits (Cat.# 34031).
试剂盒优势:
★ 快速简单的实验方案
★ 程序简单
- 易于使用的主混合反应
- 减少反应成分,简化工作台工作
★ 减少清洁过程中使用的磁珠:减少50%以上的磁珠消耗
★ 保证了较高的库转换效率
★ 低输入细胞游离DNA:从1 ng到50 ngKit advantages:
- Fast and simple protocol
- Hands-on time is around 10 minutes
- The total protocol time is only 1.5 hours
- Easy procedure based on:
- Ready-to-use master mix for easy setup of reactions
- Less reaction components to simplify bench work
- Less magnetic beads used for cleanup procedures: This can reduce more than 50% of the beads consumption
- Guaranteed high library conversion efficiency
- Low input cell-free DNA: From 1 ng to 50 ng
比较相同条件下不同样品的文库转换效率。
比较相同条件下不同样品的出库率。
产品订购:
产品名称 |
货号 |
产品规格 |
NGS cfDNA Library Prep Kit |
30029S |
non-index,24 reactions |
NGS cfDNA Library Prep Kit |
30029L |
non-index,48 reactions |
NGS cfDNA Library Prep Kit |
30031S |
index,24 reactions |
NGS cfDNA Library Prep Kit |
30031L |
index,48 reactions |
NGS cfDNA Library Prep Kit |
30033S |
unique dual index,96 reactions |
NGS cfDNA Library Prep Kit |
30033L |
unique dual index,192 reactions |
NGS cfDNA Library Prep Kit |
34031S |
index,24 reactions |
NGS cfDNA Library Prep Kit |
34031L |
index,96 reactions |