NGS 无细胞 DNA 文库制备试剂盒

BioDynami NGS cfDNA建库试剂盒

产品简介：

BioDynami开发了NGS cfDNA建库试剂盒(illumina和MGI平台)，以1 ng到50 ng的细胞游离DNA作为输入，用于构建高质量的细胞游离DNA文库。该试剂盒具有简单的工作流程和快速的程序。根据索引类型，细胞游离DNA文库的多路复用是可能的。
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.

 

NGS cfDNA建库试剂盒工作流程

游离DNA (cfDNA)的主要来源来源于血液中凋亡的造血细胞，并在血浆中发现。cfDNA的长度约为150-200 bp。来源于恶性肿瘤的循环肿瘤DNA (ctDNA)是cfDNA的一部分。cfDNA和ctDNA均可作为无创生物标志物，因为与有创组织活检相比，cfDNA提供了更好的方法。

NGS可提供全基因组水平的分子谱分析，因此已被用于液体活检领域的cfDNA和ctDNA测序。cfDNA测序的障碍之一是难以从血浆中获得数量有限的cfDNA制备文库。BioDynami的NGS cfDNA建库试剂盒可以在1.5小时内从有限的输入中轻松获得足够的文库。

The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.

NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.

illumina平台的NGS细胞游离DNA库准备试剂盒有三种index类型：

Non-index(Cat.# 30029)：没有索引。

Index(Cat.# 30031)：每个索引引物包含一个独特的6个碱基索引序列，可以用于样本识别。总共48个库复用是可能的。

Unique dual index(Cat.# 30033)：使用独特的双指数，可实现多达96个样本的无细胞DNA库复用。我们建立了一个Four-Base Difference Index System。该系统在8个碱基索引长度中至少有4个彼此不同的碱基。引物有效地减少了测序错误，如错误赋值、指数跳跃、指数污染等。

MGI平台试剂盒(Cat.# 34031)也适用上述index分类。

Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30029): Libraries do not have index.

Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48  library multiplexing is possible.

Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc.
Indexes are available for the MGI platform kits (Cat.# 34031).

试剂盒优势：

★  快速简单的实验方案

• 操作时间大约10分钟
• 总实验时间仅为1.5小时

★  程序简单

• 易于使用的主混合反应
• 减少反应成分，简化工作台工作

★  减少清洁过程中使用的磁珠：减少50%以上的磁珠消耗

★  保证了较高的库转换效率

★  低输入细胞游离DNA：从1 ng到50 ngKit advantages:

• Fast and simple protocol
  • Hands-on time is around 10 minutes
  • The total protocol time is only 1.5 hours
• Easy procedure based on:
  • Ready-to-use master mix for easy setup of reactions
  • Less reaction components to simplify bench work
• Less magnetic beads used for cleanup procedures: This can reduce more than 50% of the beads consumption
• Guaranteed high library conversion efficiency
• Low input cell-free DNA: From 1 ng to 50 ng

比较相同条件下不同样品的文库转换效率。

比较相同条件下不同样品的出库率。

产品订购：

产品名称	货号	产品规格
NGS cfDNA Library Prep Kit	30029S	non-index，24 reactions
NGS cfDNA Library Prep Kit	30029L	non-index，48 reactions
NGS cfDNA Library Prep Kit	30031S	index，24 reactions
NGS cfDNA Library Prep Kit	30031L	index，48 reactions
NGS cfDNA Library Prep Kit	30033S	unique dual index，96 reactions
NGS cfDNA Library Prep Kit	30033L	unique dual index，192 reactions
NGS cfDNA Library Prep Kit	34031S	index，24 reactions
NGS cfDNA Library Prep Kit	34031L	index，96 reactions