NGS FFPE DNA Library Prep Kit

BioDynami NGS FFPE DNA建库试剂盒

产品简介：

BioDynami开发了NGS FFPE DNA建库试剂盒(illumina和MGI平台)，从福尔马林固定，石蜡包埋(FFPE)样本中分离的10 ng到400 ng输入DNA构建高质量的FFPE DNA文库。固定所造成的DNA损伤给高质量文库的构建带来了困难。BioDynami的试剂盒经过优化，可以修复反应中受损的DNA。NGS FFPE DNA库可实现多路复用。
The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.

NGS FFPE DNA建库试剂盒流程图

FFPE标本是生物医学研究的重要资源。然而，不同的固定方法和保存条件对样本中的DNA有明显的损伤。因此，从FFPE样本中提取高质量的DNA常常是一个挑战。由于组织材料的限制和DNA的降解，FFPE DNA的产量低、质量低。

因此，低数量、低质量的FFPE DNA通常难以构建高质量的NGS文库。为了解决这一问题，BioDynami开发了NGS FFPE DNA建库试剂盒，可以从低输入的FFPE DNA样本中制作高质量的文库。

FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples.  Thus, the extraction of high quality DNA from FFPE samples is often a challenge.  Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.

As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.

illumina平台的NGS FFPE DNA建库试剂盒有三种index类型：

Non-index(Cat.# 30035)：没有索引。

Index(Cat.# 30037)：每个索引引物包含一个独特的条形码DNA(6个碱基长)，可以用来识别单个库。库的复用多达48个样本。

Unique dual index(Cat.# 30039)：基于独特的双标引系统，FFPE DNA库复用96个样本是可能的。BioDynami独特的Four–Base Difference Index System，允许我们在8基索引序列中至少有4个不同的索引。BioDynami独特的双索引引物可以有效地消除NGS错误，包括索引跳变、解复用错误、索引交叉污染、错误赋值等。唯一的双索引引物组合包括96对预混的唯一对i5和i7索引引物。

MGI平台试剂盒(Cat.# 34037)也适用于上述index分类。

Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30035): Libraries do not have index.

Index (Cat.# 30037): Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples.

Unique dual index (Cat.# 30039): FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system. Our unique Four–Base Difference Index System allows us to make indexes that have at least 4 bases different from each other in the 8-base index sequence. Our unique dual indexing primers can effectively remove NGS errors including index hopping, de-multiplexing errors, index cross-contamination, mis-assignments etc. The unique dual index primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers.

Indexes are available for the MGI platform kits (Cat.# 34037).

试剂盒优势：

★  快速简单的实验方案

★  制作文库只需1.5小时

★  10分钟的操作时间

★  操作简单

★  随时可用的主混合，减少繁琐的混合

★  减少反应组分以简化反应装置

★  净化步骤所需的磁珠较少：节省50%以上磁珠

★  低输入FFPE DNA：从10 ng到400 ng

Kit advantages:

• Fast and simple protocol
  • Making libraries in just 1.5 hours
  • 10 minutes of hands-on time
• Easy procedure
  • Ready-to-use master mix to reduce the tedious mixing
  • Less reaction components to simplify the reaction setup
• Less magnetic beads needed for the purification steps: saving more than 50% of the expensive beads
• Low input FFPE DNA: From 10 ng to 400 ng

比较相同条件下的库转换效率。输入DNA量为25 ng。

相同条件下的库产率比较。输入DNA量为25 ng。

产品订购：

产品名称	货号	产品规格
NGS cfDNA Library Prep Kit	30035S	non-index，24 reactions
NGS cfDNA Library Prep Kit	30035L	non-index，48 reactions
NGS cfDNA Library Prep Kit	30037S	single index，24 reactions
NGS cfDNA Library Prep Kit	30037L	single index，48 reactions
NGS cfDNA Library Prep Kit	30039S	unique dual index，96 reactions
NGS cfDNA Library Prep Kit	30039L	unique dual index，192 reactions
NGS cfDNA Library Prep Kit	34037S	single index，24 reactions
NGS cfDNA Library Prep Kit	34037L	single index，96 reactions