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- BioToolomics
- 英国
- 360201-50ML
- 2025年07月08日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
肼活化磁性琼脂糖介质4HF
产品名称：肼活化磁性琼脂糖介质4HF英文名称：Hydrazide-activated SepFast MAG 4HF
Product Introduction:
Hydrazide activated SepFast MAG is a magnetic agarose resin that is particularly useful for making glycoprotein-attached magnetic adsorbents.
Hydrazide-activated agarose permits the coupling of aldehyde- or ketone-containing ligands through the formation of stable hydrazone linkages. It is a particularly powerful way to immobilize glycoproteins via their carbohydrate chains while leaving critical active sites intact. It is a method called “site-directed immobilization”. For example, antibodies can be immobilized to an agarose solid support through a linkage to their glycosylated side chain that leaves their Fab region free for antigen binding.
Glycoproteins can be readily oxidized with sodium periodate to generate formyl groups on their carbohydrate chains before the coupling reaction with Hydrazide-activated SepFast MAG. It is a successful and well-documented technique. The coupling reaction is mild and easy to carry out. No toxic chemical or special equipment is required.
Hydrazide-activated SepFast MAG resins can be readily employed to make custom affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
Hydrazide activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and high chemical stability for harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated with hydrazide through a long hydrophilic spacer arm. Hydrazideactivated SepFast MAG media is supplied as an aqueous suspension.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
的浓度降低点，这也是个办法。 2)请问有没有合成过配体为FMN的亲和胶? 亲和的填料合成过20来种，你是希望用它来纯化某种脱氢酶吗，我看FMN可偶联的有限，倒是FAD有活泼的氨基好偶联，何况它们几乎是同一个辅酶，你是不是考虑把FAD连上去。当然FMN的结构上也有个仲胺，可以偶联到带长手臂的环氧活化的填料上，而溴化氰活化或别的活化的介质都不大好，因此我觉得这个没有什么问题。 此外如果是以FMN为辅酶的，那我还可以建议你试试蓝色琼脂糖凝胶，因为它的配基的结构和FMN
9.0 NaHCO3抽洗。4.偶联蛋白20ml 4B液体相当于一半的固相载体。事先将需偶联的抗原（或抗体）蛋白200mg置于0.1Mol/L pH 9.0 NaHCO3液中透析数小时(一般偶联量为10~30mg/g载体)。5.将活化的琼脂糖迅速倒入蛋白液中（在1.5min内，从抽洗到偶联），4℃缓慢搅拌过夜，使蛋白与活化的琼脂结合。6.在烧结玻璃漏斗中用200ml偶联缓冲液（0.1mol/L碳酸氢钠，0.5mol/L氯化钠，pH8.3）洗偶联介质一次。7.转移偶联介质至含100ml阻断缓冲液（1mol/L乙醇
粒子的表面(或孔内)即可制备亲和吸附介质，该固体粒子通常称为配基的载体。因此，亲和吸附介质又称亲和载体。作为载体的固体粒子应满足下列要求:(1)具有亲水性多孔结构，无非特异性吸附，比表面积大;(2)物理和化学稳定性高，有较高的机械强度，使用寿命长;(3)含有可活化的反应基团，用于亲和配基的固定化;(4)粒径均一的球形粒子。常用的亲和层析载体有:琼脂糖凝胶和交联琼脂糖凝胶、聚丙烯酰胺凝胶、葡聚糖凝胶、聚丙烯酰胺—琼脂糖凝胶、纤维素、多孔玻璃等。其它用于载体的物质还有:聚苯乙烯、淀粉珠、壳聚糖、硅胶
