- ￥500
- BioToolomics
- 英国
- 310201-1G
- 2025年07月08日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
1G;10G;100G
产品名称：CNBr活化磁性琼脂糖凝胶4HF
英文名称：CNBr-activated SepFast MAG
Product Introduction:
CNBr activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.
CNBr-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
The application areas cover immobilization of proteins, peptides and nucleic acids.
Product Properties:
CNBr activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible so that low pH and certain organic solvents of high concentrations can be applied.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized. CNBr-activated SepFast MAG media is supplied as a freeze-dried powder stabilized with additives.
Product Series:
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
浓度大于0.05Mol/L时，对蛋白质几乎没有非特异性吸附。琼脂糖凝胶极易被溴化氢活化，活化后性质稳定，能经受层析的各种条件，如0.1Mol/L NaOH或1Mol/L HCl处理2h~3h及蛋白质变性剂7Mol/L尿素或6Mol/L盐酸胍处理，不引起性质改变，故易于再生和反复使用。 琼脂糖凝胶微球的商品名为Sepharose，含糖浓度为2%、4%、6%时分别称为2B、4B、6B。因为Sepharose 4B的结构比6B疏松，而吸附容量比2B大，所以4B应用最广。 （三）试剂与配制
性吸附。琼脂糖凝胶极易被溴化氢活化，活化后性质稳定，能经受层析的各种条件，如0.1Mol/L NaOH或1Mol/L HCl处理2h~3h及蛋白质变性剂7Mol/L尿素或6Mol/L盐酸胍处理，不引起性质改变，故易于再生和反复使用。 琼脂糖凝胶微球的商品名为Sepharose，含糖浓度为2%、4%、6%时分别称为2B、4B、6B。因为Sepharose 4B的结构比6B疏松，而吸附容量比2B大，所以4B应用最广。 （三）试剂与配制 1．Sepharose 4B 2．CNBr（剧毒） 3．
粒子的表面(或孔内)即可制备亲和吸附介质，该固体粒子通常称为配基的载体。因此，亲和吸附介质又称亲和载体。作为载体的固体粒子应满足下列要求:(1)具有亲水性多孔结构，无非特异性吸附，比表面积大;(2)物理和化学稳定性高，有较高的机械强度，使用寿命长;(3)含有可活化的反应基团，用于亲和配基的固定化;(4)粒径均一的球形粒子。常用的亲和层析载体有:琼脂糖凝胶和交联琼脂糖凝胶、聚丙烯酰胺凝胶、葡聚糖凝胶、聚丙烯酰胺—琼脂糖凝胶、纤维素、多孔玻璃等。其它用于载体的物质还有:聚苯乙烯、淀粉珠、壳聚糖、硅胶
