- ￥480 - 780
- Solarbio
- 北京索莱宝科技有限公司
- K200057M
- 2025年12月30日
- WB IHC IF
- Mouse
- H R M Mk Dg Ch Hm Rb Pg Sh Insect Yeast
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human GAPDH
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 适应物种：
H R M Mk Dg Ch Hm Rb Pg Sh Insect Yeast
- 库存：
99
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
WB IHC IF
- 靶点：
GAPDH
- 抗体英文名：
Anti-GAPDH Monoclonal antibody
- 抗体名：
GAPDH
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥780.0 |
| 英文名称 | Anti-GAPDH Monoclonal antibody |
| 中文名称 | 3-磷酸甘油醛脱氢酶抗体 |
| 别名 | GAPDH |
| 背景资料 | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. |
| Swiss Prot | P04406 |
| Gene ID | 2597 |
| 亚细胞定位 | Cytoskeleton Cytosol Nucleus |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | IgG1 |
| 蛋白分子量 | 36kDa |
| 稀释比例 | WB 1:3000-8000. IHC 1:100-300. IF 1:100-200. |
| 免疫原 | Recombinant protein of human GAPDH |
| Public Immunogen Range | Recombinant protein of human GAPDH |
| 纯化方法 | Affinity purification |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 性状 | 液体 |
| 交叉反应 | Human Dog Rabbit Pig Mouse Rat |
| 应用 | WB IF IHC |
| 规格 | 50ul 100ul |
| 单位 | 支 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图
Western blot analysis with GAPDH antibody diluted at 1:3000;Lane: HeLa
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，但是进口品牌如Abcam、Millipore、CST等价格偏高，一般在￥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体，分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody （货号E021010 ）是小鼠源的单抗，其效价很高，厂商推荐的WB起始稀释比率为1:1000，实际应用过程中，有使用1:10000或更高的报告，远远高于此稀释比率，说明其免疫效价很高，另外，其检测种属也很多，包括人、牛、猪、小鼠
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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