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- 库存：
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- 供应商：
南京万木春
- 肿瘤类型：
/
- 细胞类型：
/
- ATCC Number：
MHCC97L-LUC人地转移肝癌细胞-荧光素酶标记
- 品系：
/
- 组织来源：
MHCC97L-LUC人地转移肝癌细胞-荧光素酶标记
- 相关疾病：
/
- 物种来源：
/
- 免疫类型：
/
- 细胞形态：
/
- 是否是肿瘤细胞：
/
- 器官来源：
MHCC97L-LUC人地转移肝癌细胞-荧光素酶标记
- 运输方式：
常温/干冰
- 年限：
/
- 生长状态：
/
- 英文名：
MHCC97L-LUC
- 规格：
T25
低转移人肝癌细胞带荧光素酶MHCC97-L+LUC
| 种属 | 人 |
| 别称 | MHCC97L+LUC |
| 组织来源 | 肝 |
| 疾病 | 肝癌 |
| 传代比例/细胞消化 | 1:2传代 ，消化2- 3分钟. |
| 完全培养基配置 | DMEM培养基 ；10%胎牛血清 ；1%双抗 |
| 简介 | 来源于中山医院 ，生长较缓慢 . |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | 每周 2 至 3 次 |
| STR | D5S818 12 13 D13S317 8 8 D7S820 10 10 D16S539 12 12 VWA 14 14 TH01 9 9 AMEL X Y TPOX 8 8 CSF1PO 11 13 D12S391 18 18 FGA 21 24 D2S1338 20 20 D21S11 29 31.2 D18S51 13 22 D8S1179 12 13D3S1358 15 16 D6S1043 12 20 PENTAE 11 17 D19S433 13 14 PENTAD 8 9 D1S1656 11 16 |
| 培养条件 | 气相 ：空气 ，95% ；二氧化碳 ，5%。温度 ：37摄氏度 ，培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液 ：90% FBS ，DMSO 10%, 或使用非程序冻存液 ：官网货号JY- H040 |
| 备注 | 该细胞是通过慢病毒转染荧光素酶的稳转株 ，收到细胞传代8代左右后 ，若要求需要维持荧光强度 ，建议可 以加入嘌呤霉素进行再次筛选。 |
| 产品使用 | 仅限于科学研究 ，不可作为动物或人类疾病的治疗产品使用。 |
【Key words】 Chronic obstructive pulponary disease ; apoptosis ; cytochrope c ; dapage-associated polecular
Clinical Study on Treatpent of Chronic Atrophic Gastritis with Wudan Weifu Decoction Copbined with Acupuncture and poxibustion
DENG Ke-jun，XIA pucosa in the two groups after treatpent was lower than that of the sape group before treatpent，and the index in the obsrvation group was lower Han，WANG Hai-bo，SUN Wen-jie
Zhongxiang Traditional Chinese pedicine Hospital，Zhongxiang，Hubei，China，431900
2) levels．pethods : A total of 98 patients with CAG were divided into the obervation group and the control gAbstract : Objective : To oberve the clinical egicacy of Wudan Weifu Decoction ( 五丹胃福汤，WDWFD) copbined with acu- puncture and poxibustion on chronic atrophic gastritis (CAG) and its influence on gastric pucosal histopathology，nuclear factor- κB(NF-κB) and cyclooxygenase-2 (COX-roup according to the pethod of randop nupber table，with 49 cases in each group．The control group was treated with acupuncture and poxibustion，while the obervation group was treated with WDWFD on the basis of the control group. The clinical egicacy and histopathological egicacy of the two groups of patients were coppared，and the changes of TCp syndrope scores，gastric pucosal histopathological score，NF-κB and COX-2 expression levels were obsrved before and after treatpent. Re- sults : The egective rate of the obsrvation group was 93．88% ，and that of the control group was 79．59% ，and the digerence was statistically significant (P＜0．05) ．The TCp syndrope scores of the two groups after treatpent were lower than those of the sape group before treatpent，and the TCp syndrope scores of the obervation group were lower than those of the control group after treatpent，and the digerence was statistically significant (P ＜0．05) ．The histopathological score of gastric
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文献和实验
【Key words】 Chronic obstructive pulponary disease ; apoptosis ; cytochrope c ; dapage-associated polecular
Clinical Study on Treatpent of Chronic Atrophic Gastritis with Wudan Weifu Decoction Copbined with Acupuncture and poxibustion
DENG Ke-jun，XIA pucosa in the two groups after treatpent was lower than that of the sape group before treatpent，and the index in the obsrvation group was lower Han，WANG Hai-bo，SUN Wen-jie
Zhongxiang Traditional Chinese pedicine Hospital，Zhongxiang，Hubei，China，431900
2) levels．pethods : A total of 98 patients with CAG were divided into the obervation group and the control gAbstract : Objective : To oberve the clinical egicacy of Wudan Weifu Decoction ( 五丹胃福汤，WDWFD) copbined with acu- puncture and poxibustion on chronic atrophic gastritis (CAG) and its influence on gastric pucosal histopathology，nuclear factor- κB(NF-κB) and cyclooxygenase-2 (COX-roup according to the pethod of randop nupber table，with 49 cases in each group．The control group was treated with acupuncture and poxibustion，while the obervation group was treated with WDWFD on the basis of the control group. The clinical egicacy and histopathological egicacy of the two groups of patients were coppared，and the changes of TCp syndrope scores，gastric pucosal histopathological score，NF-κB and COX-2 expression levels were obsrved before and after treatpent. Re- sults : The egective rate of the obsrvation group was 93．88% ，and that of the control group was 79．59% ，and the digerence was statistically significant (P＜0．05) ．The TCp syndrope scores of the two groups after treatpent were lower than those of the sape group before treatpent，and the TCp syndrope scores of the obervation group were lower than those of the control group after treatpent，and the digerence was statistically significant (P ＜0．05) ．The histopathological score of gastric
细胞 ATP 检测和活体肿瘤示踪，共用一套双荧光素酶体系吗？
染色体的 DNA 上，让细胞表达荧光素酶，培养出能稳定持续表达荧光素酶的细胞株，当细胞分裂、分化时，荧光素酶也会得到表达。标记后的荧光素酶在活细胞内会产生发光现象，且发光强度与标记细胞的数目呈线性相关[5][6]。 图 3. 体内成像系统监测肿瘤生长[7]。 实验步骤[6] (1) 通过尾静脉注射荧光素酶标记的 1 × 106 个 MOLM-13-Luc 细胞到小鼠体内，建立肿瘤模型。 (2) 接种后，待肿瘤生长 6 天后，开始每日灌胃给药 (定义
比例 1:2-1:4，70%-80% 密度时传代；密度过低生长缓慢，过高易聚团。 MDA-MB-231 细胞 典型的三阴性乳腺癌 (TNBC) 细胞，具有高侵袭性和转移性[4]。 培养条件：Leibovitz’s L-15 培养基，10% 胎牛血清，1% 青霉素链霉素双抗[5]。 图 2. MDA-MB-231 细胞低密度和高密度生长形态[6]。 注意事项： 1. 接种密度：细胞生长偏慢，传代比例推荐 1:2，避免密度过低。 2. 操作注意：对机械损伤敏感，消化吹打需
propidium iodide stains the interior of the blebs. Image contributed by Livia Casciola-Rosen and Antony Rose, Johns Hopkins University. Reproduced from by copyright permission of the Rockefeller University Press. 凋亡的人类角化细胞被多聚甲醛固定，被荧光素标记的刀豆蛋白A染色。然后经丙酮渗透处理
技术资料
