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描述
4-Nitro blue tetrazolium chloride, crystals
质量水平
100
形式
crystals
包装
pkg of 5 g
manufacturer/tradename
Roche
运输
wet ice
储存温度
2-8°C
SMILES string
[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI
1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key
FSVCQIDHPKZJSO-UHFFFAOYSA-L
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. It gives a bright blue color, although the reaction is quite slow, even at 37O C. NBT added to X-gal gives an intense purple color which develops much more rapidly. PMS will increase this reaction rate even further. Tetrazolium red makes the product look
Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations
, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nitro Blue Tetrazolium (NBT). NBT is converted
Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
, such as Nitro Blue Tetrazolium (NBT), that was converted to an insoluble, colored compound at the site of hybridization, thus facilitating visualization of the hybridized probe.
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