- $180
- UBPBIO
- Y3011
- 2026年01月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
保质期内
- 供应商：
北京美科美生物技术开发有限公司
- 规格：
100 µg
| Product Name: | Anti-Ubiquitin (P4D1) |
|---|---|
| Also Known As: | Anti-Ub |
| Catalog No.: | Y3011 |
| Size: | 100 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | Recognizes ubiquitin in a wide range of species |
| Source: | Immunogen: Full length bovine ubiquitin |
| Stock: | lyophilized powder |
| Concentration: | lyophilized powder |
| Quality Assurance: | Guaranteed for detecting 5 ng endogenous ubiquitin or polyubiquitin chains in whole cell or tissue lysates at an antibody dilution of 1:1,000. |
| Storage: | 1) Store the lyophilized antibody desccated at -20°C. 2) After reconstitution, store the antibody at -20°C. Aliquot is not necessary. The quality of the antibody is guaranteed for one year after puchasing if stored correctly. |
| PDF Data Sheet: | PDF Datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge) Figure 1.Western blot applications Anti-Ubiquitin (P4D1) was used at a 1:500 dilution. Bovine thymus ubiquitin was run as follows; Lane 1-50 ng, Lane 2-25 ng. Lane 3-12.5 ng, Lane 4-6.25 ng, Lane 5-3.12 ng. Lanes 6 & 7 represent 20 μg of Swiss 3T3 cell lysate from cells treated for 5h with 10 µM MG-132 (Lane 6) or untreated cells (Lane 7). Arrow indicates free ubiquitin band (8 kD), higher molecular weight bands are ubiquitinated proteins.  Figure 2. IF of HeLa cells HeLa cells were stained and visualized by fluorescence microscopy. Ubiquitin targeted cytoplasmic and nuclear proteins and free ubiquitin were stained using Anti-Ubiquitin (P4D1) at 1:500 dilution. |
| Shipping Method: | Room temperature shipping |
| References: | N/A |
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文献和实验Preparation of Carboxypeptidase Y and Its Properties
speed centrifuge; Refrigerator; Constant temperature water boiler; G-3 Core sand funnel (500mL); Fraction collector; Constant speed pump; Disk electrophoresis apparatus; Chromatography column (2.6×50cm) (1.0×100cm) (0.6×30cm); Dialytic bag.
Studies of the Ubiquitin Proteasome System
( protocol 33 ), or mouse E1 ( protocol 34 ) 100 mM HEPES, pH 7.5 10× thiolester buffer (see recipe ) [32 P]ubiquitin ( protocol 26 ) or 10 µg/µl “cold
Characterization of Neuropeptide Y (NPY) Receptors
nM unlabeled pNPY 13‐36 . g Assayed in the presence of 100 nM unlabeled p[Leu31 Pro34 ]NPY
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