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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
−20°C
- CAS号:
见产品外包装
- 英文名:
NBT 4-Nitro blue tetrazolium chloride, solution
- 库存:
需确认
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- 规格:
3 mL
NBT
4-Nitro blue tetrazolium chloride, solution
General description
NBT是一种发色和可溶性物质。它是碱性磷酸酶(AP)的底物,并在其作用下转化为靛蓝/紫色染料。
Application
• 免疫组织细胞化学
• 蛋白质免疫印迹
• Southern印迹
• Northern印迹
• 菌落和噬菌斑杂交
• 原位杂交
Physical form
该溶液(100 mg/ml)的溶剂为70%二甲基甲酰胺(DMF) (v/v)。NBT溶液的颜色介于黄色和棕色之间,具体取决于所用批次。
注意:颜色不影响试剂的质量或功能。在某些情况下,可能产生沉淀,在37℃下短暂加热即可使沉淀溶于溶液。
Preparation Note
工作溶液:染色溶液的制备
• 临用前配制新鲜溶液!对于除DIG标记系统以外的所有应用:将50 μl NBT溶液和37,5 μl BCIP加至10 ml 0.1 M Tris-HCl中,pH 9.5 (20 °C),含0.1 M NaCl和0.05 M MgCl2。
• 对于DIG标记系统应用:将50 μl NBT溶液和37,5 μl BCIP加至10 ml 0.1 M Tris-HCl中,pH 9.5 (20 °C),含0.1 M NaCl
• 注:请勿在DIG检测缓冲液中加入MgCl2 ,否则在检测过程后膜上可能会产生斑点背景。碱性磷酸酶不需要Mg2+。
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. It gives a bright blue color, although the reaction is quite slow, even at 37O C. NBT added to X-gal gives an intense purple color which develops much more rapidly. PMS will increase this reaction rate even further. Tetrazolium red makes the product look
Measurement of Chloride Movement in Neuronal Preparations
, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand?gated chloride movement in vesicular structures called synaptoneurosomes (also referred to as microsacs), in cultured neurons
Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations
, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nitro Blue Tetrazolium (NBT). NBT is converted
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