Human CYFRA21-1 (诊断原料,肿瘤标志物）

Recombinant Human CYFRA21-1 

 

Catalog #:  DRA08        Derived From: E.coli   

 

Description:

Recombinant Human CYFRA21-1 is produced by E.coli expression system and the target gene encoding Met1-Leu400 is expressed with a 6His tag at the N-terminus.

Accession:  P08727

Names(Known as):CK-19, CK19, Cytokeratin-19, K19, K1CS

 

Quality Control:

Mol Mass:  46.3kDa              AP Mol Mass:  40-50kDa, reducing conditions

Purity:  Greater than 95% as determined by reducing SDS-PAGE.

 

Formulation:

Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 10% glycerol, pH8.0.

 

Shipping:

The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.

 

Storage:

Reconstituted protein solution should be stored at ≤ -20°C.

 

Purification: 
Affinity purification chromatography.

 

Immunoreactivity: 
N.A.

 

Application:
Immunogen, calibrator or standard.

 

Background:

CYFRA 21–1 is a fragment of cytokeratin (CK) 19. CKs, which are now called keratins, are the principal structural elements of the cytoskeleton (keratin filaments) of epithelial cells, including bronchial epithelial cells, and have been classified into 20 subtypes based on differences in the molecular mass and isoelectric point as determined by 2-dimensional electrophoresis.The CK expression patterns in tissues are well-maintained even during the process of transformation of the tissue from normal to tumor tissue. Accelerated CK19 degradation occurs in neoplastically transformed epithelial cells as a result of increased protease activity of caspase 3, a regulator of the apoptosis cascade, and fragments are released into the blood. This results in an increase of the blood CYFRA 21–1 values, because CK19 fragments are recognized by two monoclonal antibodies.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY