Human Lp-PLA2 Antibody (4E11)

Human Lp-PLA2 Antibody (4E11)

 

Catalog #: DA056

 

Spcificty: Antibody recognizes Human Lp-PLA2

 

Isotype: Mouse IgG1

   

Source: Cultrued in vitro under conditions free from animal derived components.

 

Product buffer solution: PBS, pH 7.4

 

Shelf life and storage: 24 months from manufacturing at <-20 °C; 1 month, 2 to 8 °C under sterile conditions.

 

Analyte Description: Platelet-Activating Factor Acetylhydrolase (PAFAH) is a secreted enzyme which belongs to the AB hydrolase superfamily and Lipase family and catalyzes the degradation of platelet-activating factor to biologically inactive products. PAFAH is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. PAFAH has been implicated in the development of atherosclerosis and has also been identified as a marker for cardiac disease. PAFAH might have a major physiologic effect in the presence of inflammatory bodily responses. PAFAH alters the action of PAF by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. PAFAH has specificity for substrates with a short residue at the sn-2 position.

 

PARAMETERS TESTED ON EACH LOT

Product appearance: Liquid, may turn slightly opaque during storage

 

Product concentration: 5.0 mg/ml (+/- 10 %)

 

Immunoreactivity: 80–120 % compared to the reference sample in an ELISA test.

 

Purity: ≥95 %

 

Bioactivity-BLI:

Loaded Human Lp-PLA2 Antibody (4E11)-mFc(Cat#DA056) on AMC Biosensor, can bind Human PLA2G7-His(Cat#DC624) with an affinity constant of 0.015 nM as determined in BLI assay.		Loaded Human PLA2G7-His(Cat#DC624) on HIS1K Biosensor, can bind Human Lp-PLA2 Antibody (4E11)-mFc(Cat#DA056) with an affinity constant of <10-3 nM as determined in BLI assay.

 

Product stability: 

 

TEMPERATURE,	TIME	RESULT
+37 °C,	7 days	OK

 

Pair recommendations:

 

CAPTURE ANTIBODY	DETECTION ANTIBODY
DA056	DA057
DA057	DA056
DA056	DA059
DA059	DA056

Please note that pair recommendations are based on results obtained by our laboratory. Equally good results may be obtained using other pairs and therefore these recommendations are only indicative.

 

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY