Human Antibody Gene Libraries

The quality of antibodies selected from antibody gene libraries, in particular their affinity and the number of different clones per antigen, is a direct function of library diversity. Here, the optimization of every single step during library construction is of utmost importance for the quality. This chaper describes the generation of human naive or immune scFv antibody gene libraries by a two step cloning strategy. The method has already been proven for the generation of very high quality naive and immune libraries.