Retrovirus service

Creative Biogene is pioneering in the development of retroviral technology with years of experience in retroviral services. Creative Biogene's custom retrovirus production is a highly efficient approach to deliver genes of interest for stable expression into a broad range of dividing mammalian cells and it aims to provide valuable research tools and services in molecular biology.

Retroviruses are negative single-stranded RNA viruses in which tRNA serves as a primer for the mRNA synthesis. The mRNA is then reverse transcribed into DNA, which is then integrated into the chromosome at a multiplicity of sites, particularly those that are transcriptionally active. Retroviral vector-mediated gene transfer has been central to the development of gene therapy. Retroviruses have several distinct advantages over other vectors, especially when permanent gene transfer is the preferred outcome. The most important advantage that retroviral vectors offer is their ability to transform their single-stranded RNA genome into a double stranded DNA molecule that stably integrates into the target cell genome. This means that retroviral vectors can be used to permanently modify the host cell nuclear genome. Creative Biogene retrovirus vectors are produced in compliance with GLP standards. The retroviral vector provides high-level expression of a variety of inserted genes, like cytokines, receptors, enzymes, and growth factors, following non-selective infection of cells.

Creative Biogene offers custom retrovirus construction services for your scientific research as follows:
• Construction of ordered retrovirus vector (gene expression and shRNA expression).
• Production of a retrovirus producer cell line by transfection.
• Production of viral supernatant by transient transfection as well as assay for replication competent
retrovirus.
• Quality control assays including quantification of genome copies and quantification of retroviral
vector infectivity.