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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Bst DNA Polymerase, Full Length (with dNT P)
- 规格:
250U
目录号:E014-01A
产品描述:
Bst DNA聚合酶来源于Bacillus stearothermophilus,具有 5'→3' DNA聚合酶活性和双链特异的5'→3'核酸外切酶活性,但缺失3'→5'核酸外切酶活性。本制品利用基因重组技术,在大肠杆菌中进行表达后经多次纯化分离而得。
产品用途:
1)富含GC 序列的DNA 序列测定;
2)微量(纳克量)DNA 模板的快速测序等。
注意事项:
1)Bst DNA聚合酶不具有3´→5´外切酶活性。
2)长期贮存需加入100μg/ml BSA 或0.1%Triton X-100。
3)建议反应温度不要超过70℃。
4)不能用于热循环测序或PCR。
保存温度:
-20℃。
参考文献:
A Loop-Mediated Isothermal Amplification Integrated G-Quadruplex Molecular Beacon (LAMP-GMB) Method for the Detection of Staphylococcus aureus in Food[J]. Jingguo Xu et al. Food Analytical Methods. 2018.
For research use only.
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文献和实验Bst-catalyzed radiolabeled DNA sequencing
Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions are modified from those presented earlier by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios in the termination mixes
nuclear RNA promoter-based parent vector using a single-stranded inverted repeat DNA and Bst DNA polymerase. The shRNA expression plasmids constructed by this method were confirmed to promote efficient RNA interference knockdown in silkworm cell lines
Optimal arbitrary primer length for Differential Display(图)
for PCR amplification (Williams et al, 1991, Nucleic Acids Res. 18:6531), probably due to the minimum contact surface between Taq polymerase with the double-stranded DNA template. Initially, arbitrary decamers (10mers) were employed for differential
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