- ￥1380 - 9300
- SBS
- BST-1600
- 2026年03月25日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京赛百盛基因技术有限公司
Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely thermostable reverse transcriptase (65°C tolerant), which is suitable for the isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform isothermal amplification of DNA templates.
Our Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: BST-1600 (for 1,600U)
Cat. No.: BST-16k (for 16KU)
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system. Please see Bst DNA/RNA Polymerase (glycerol-free) for more details.
Description
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase.
Bst DNA/RNA Polymerase is suitable for isothermal amplification reaction of both DNA and RNA templates, which can detect low-sensitivity nucleic acid templates with great efficiency and specificity. Besides, with a special preparation process, this enzyme has a fast amplification rate and high tolerance to impurity. Since our Bst DNA/RNA Polymerase is extremely thermostable and also provides sensitive reverse transcriptase activity, it is reported to have higher sensitivity at high Ct values.[1]
[1] Lu S, Duplat D, Benitez-Bolivar P, León C, Villota SD, Veloz-Villavicencio E, et al. (2022) Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application. PLoS ONE 17(5): e0268340. https://doi.org/10.1371/journal.pone.0268340
Contents
1,600U
- DNA/RNA isothermal amplification
- GC-rich rapid sequencing
- Rapid sequencing of micro-template DNA
Strong Recognition Ability to dUTP
Bst DNA/RNA polymerase has a strong recognition ability to dUTP. The dTTP needed for amplification reaction can be completely replaced by dUTP, so the amplification products all contain dUTP. By adding our Heat-Labile Uracil DNA Glycosylase (HL-UDG), aerosol pollutants will be completely removed in the initial reaction stage. The HL-UDG can be later inactivated irreversibly within 3 min at 65°C, which not only eliminates the pollutants but also ensures the normal amplification of nucleic acid. Therefore, the false positive caused by aerosol pollution in the reaction can be greatly reduced.
Comparison
Storage Conditions
Store the components at -20°C. Avoid multiple freeze-thaw cycles.
Additional Notes
- We provide robust and reliable solutions for the study of various diseases (African Swine Fever, COVID-19, etc) based on Bst DNA/RNA Polymerase, please contact us or email tech@sbsbio.com for more details.
- For isothermal amplification of DNA only, please see Bst DNA Polymerase.
- For the lyophilized version, please see PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads.
Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021. Please visit:
https://www.science.org/doi/10.1126/science.acx9701
Instruction: Protocol
Viewpoint
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验nuclear RNA promoter-based parent vector using a single-stranded inverted repeat DNA and Bst DNA polymerase. The shRNA expression plasmids constructed by this method were confirmed to promote efficient RNA interference knockdown in silkworm cell lines
Bst-catalyzed radiolabeled DNA sequencing
Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions are modified from those presented earlier by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios in the termination mixes
RNA 依赖性 DNA 聚合酶 RNA dependentDNA polymerase
RNA 依赖性 DNA 聚合酶 RNA dependentDNA polymerase 为依赖于 RNA合成 DNA的酶，亦称逆转录酶（反向转录酶， reverse transcriptase）。系包含在各种逆向病毒粒子中的酶。以病毒单链 RNA为模板合成与模板互补的核苷酸序列的 DNA。 1970年由坦明及巴尔的摩（ H.M.Temin et al.和 D.Baltimore）各自独立的发现病毒的 RNA，首先通过此酶合成 RNA－ DNA复合体，进而由这个复合体合成
技术资料暂无技术资料 索取技术资料
