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- 2025年07月10日
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- 保存条件：
-20
- 库存：
大量
- 供应商：
天威
- 规格：
1.25ml/1.25mlx8
| 规格： | 产品价格： | ￥350 | |
|---|---|---|---|
| 规格： | 1.25ml | 产品价格： | ￥350 |
| 规格： | 1.25mlx8 | 产品价格： | ￥2600 |
使用:
●液体可用于细胞培养。
●用于筛选转化株的浓度根据pH值和盐浓度改变。
●pH越高，盐浓度越低活性越大。
●普通大肠杆菌菌株筛选可在25＆micro；g／ml 浓度的低盐LB琼脂培养基中进行，pH调至7.5。
●酵母筛选：取决于种属和培养基，S。cerevisiae Sh ble转化株在50＆micro；g／ml浓度的YPD培养基中筛选，pH7.0.304015.
●动物细胞筛选：根据不同细胞系从100到750＆micro；g／ml。
●植物细胞筛选：根据不同的细胞种类和培养基从50到250＆micro；g／ml。
Invitrogen相关资料如下:
Cat. No. R250-01
™ Selection Reagent
Description
Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin™ is prevented from binding and cleaving the cellular DNA. Zeocin™ is effective on most aerobic cells and can be used for selection in and insect cell lines, yeast, and bacteria. The amount of ™ required for selection will vary between 50-2000 ug/ml (typically 300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
™ is a trademark of CAYLA.
The ble resistance gene is the subject of one or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only. For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.
General Specitions
Concentration: 100 mg⁄mL
Brand:
Form: Liquid
Product Size: 1 g
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation
Bacterial Selection
Contents & Storage
™ is provided in solutiona concentration of 100 mg/ml. The 1 gram size is supplied in 8 vials. The 5 gram size is supplied in one vial. Store ™-20°C in the dark. ™ is guaranteed stable for 6 months when properly stored.
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文献和实验肺纤维化模型简介： 博莱霉素是具有多种抗肿瘤作用的多组分复合抗生素，其毒副作用之一是引起肺纤维化。由于病理组织学改变与人类肺纤维化最为接近，故被广泛用于诱导肺纤维化模型。 气管内给药是最为常用的给药途径，方法是是BLM进入动物气管后立即通过直立旋转等手段，使药物均匀分布于肺组织，导致肺部病变发生PF。气管给药可以是一次性的，也可以是重复多次的。其中使药物进入动物气管内有三种方法：1.直接支气管插管，然后滴入BLM。2.麻醉动物动物，手术剖开颈部
轮枝链霉菌（ Streptomyces verticillus）产生的抗肿瘤的抗菌物质，由梅沢浜夫等（ 1965）分离出来的。可分为 A、 B两大类， A类还可细分为 5种。在化学结构上，是含 2-3个硫原子的多肽与铜螫合。抗肿瘤作用是基于它可抑制 DNA合成，抑制作用包括切断 DNA和抑制 DNA多聚酶的合成．据称对鳞状上皮细胞癌尤其有效。
Multiple gene expression can be introduced in a yeast strain with using only two markers by means of the two new vectors described, the expression vector pB3 PGK and the CRE recombinase vector pCRE3. The pB3 PGK has a zeocin-selectable marker
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