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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Recombinant Human IL-2
- 规格：
10ug
Source: Escherichia coli.
Molecular Weight: Approximately 15 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
Quantity: 10ug/50ug/1000μg
Purity: >97% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.5.
Endotoxin: Less than 1EU/μg of rHuIL-2 as determined by LAL method.
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文献和实验直接IM―PCR (以rhIL-3的检测为例) 【试剂与配制】 TBS：20mmol/L Tris-HCl（pH7.4），150mmoI/L NaCL 封闭液：1.5％ BSA （用TBS配制） 抗hIL-3单抗 生物素化羊抗鼠IgG 亲合素 DNA指示分子(生物素―AdAT) PCR反应液：5×PCR缓冲液10μl，dNTP 4μl，引物4μl，EP-DNA聚合酶0.5μl,蒸馏水31.5μl 【操作方法】 (1) 用TBS稀释rhIL-3至6.0μg
Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL
culture media containing recombinant human stem cell factor (rhSCF), rhIL-6, and rhIL-3 (first week only). Weekly hemidepletions and the removal of adherent cells and/or debris enables the investigator to obtain HMC cultures, identified by Wright-Giemsa
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