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- 规格：
10ug
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Murine M-CSF (10ug)
产品描述：
一、基础标识与同源名称
- 同义名称：Macrophage Colony Stimulating Factor、CSF-1、MGI-IM
- 基因与蛋白编号：Gene ID:12977、Accession Number:P07141
- 表达来源：大肠杆菌（E.coli）
二、分子核心特征
- 分子结构：为36.4 kDa 同源二聚体蛋白，由两个各含 156 个氨基酸的多肽亚基组成，提供明确的单体氨基酸序列。
- 物种交叉反应性：人源 M-CSF 可在鼠源体系中发挥作用，但鼠源 M-CSF 对人类细胞无活性，存在物种特异性。
三、生物学功能与应用
- 核心调控作用：作为强效造血因子，由淋巴细胞、单核细胞、成纤维细胞、内皮细胞、成肌细胞、成骨细胞等多种细胞产生，是血液单核细胞、组织巨噬细胞及其祖细胞的增殖、分化、存活关键调控因子。
- 其他生理作用：可调节皮肤厚度、影响生育能力。
- 临床应用：用于治疗感染、恶性肿瘤、动脉粥样硬化，能促进骨髓移植后的造血功能恢复。
四、产品质控与活性指标
- 纯度：经 SDS-PAGE 凝胶和 HPLC 分析，纯度 **≥98%**。
- 内毒素水平：＜0.1 ng/μg 蛋白（即＜1 EU/μg），质控标准严格。
- 生物活性：通过 M-NFS-60 细胞的剂量依赖性增殖刺激检测，半数有效浓度（ED50）≤1.0 ng/ml，对应比活性 **≥1×10⁶ units/mg**。
M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits.
Source:E.coli
Synonyms:Macrophage Colony Stimulating Factor, CSF-1, MGI-IM
AA Sequence (monomer):MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Purity:≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Calculated Molecular Weight:36.4 kDa
Accession Number:P07141
Gene ID:12977
Endotoxin:Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
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文献和实验[1]Xiao H, Du X, Tao Z, et al. Taurine Inhibits Ferroptosis Mediated by the Crosstalk between Tumor Cells and Tumor-Associated Macrophages in Prostate Cancer. Adv Sci (Weinh). 2024;11(3):e2303894. doi:10.1002/advs.202303894
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