- ￥720 - 14400
- 近岸蛋白(Novoprotein)
- C028
- 2025年09月17日
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- 规格：
10μg/50μg/500μg/1mg
| 规格： | 10μg | 产品价格： | ￥720.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2000.0 |
| 规格： | 500μg | 产品价格： | ￥10080.0 |
| 规格： | 1mg | 产品价格： | ￥14400.0 |
产品描述
Recombinant Mouse Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed with a 6His tag at the C-terminus.
蛋白编号
P97466
产品别称
Noggin; Nog
分子量
23.9 KDa
表观分子量
30 KDa, reducing conditions
剂型描述
Lyophilized from a 0.2 μm filtered solution of PBS, 5mM EDTA, 5% Trehalose, pH 7.4.
内毒素
< 1 EU/µg as determined by LAL test.
纯度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
纯度-SEC-HPLC
Greater than 95% as determined by SEC-HPLC. (Regularly tested)
生物活性-Cell Based Assay
Mouse intestinal organoids were cultured with EGF (C029), Noggin (C028) and R-spondin 1 (CX83). After five days of culture, the organoids showed good morphology and germination rate.
Mouse Gastric organoids were cultured with EGF (C029), Wnt3a (C22R), Noggin (C028) , R-spondin 1 (CX83) and FGF-10 (CR11). The organoids showed good morphology.
Mouse Colon organoids were cultured with EGF (C029), Wnt3a (C22R), Noggin (C028) , R-spondin 1 (CX83) and FGF-10 (CR11). The organoids showed good morphology.
复溶
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
运输条件
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
保存条件
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
产品背景
Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.
仅供科研或生产使用，不可直接应用于人体。
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文献和实验该产品被引用文献
[1]Establishment of human minor salivary gland organoids in laminin-GelMA hydrogel from healthy individuals and Sjögren’s disease patients;Enhang Lu, Jiaying Qian, Wei Liang, Haibo Xiang, Pengbing Ding, Mengying Jin, Zhiyu Lin, Yujie Chen, Zheng Wang, Xin Huang, Zhixuan Sun, Bolin Pan, Zhenmin Zhao;2024/12/04;CHEMICAL ENGINEERING JOURNAL
[2]Indole-3-aldehyde Alleviates High-Fat Diet-Induced Gut Barrier Disruption by Increasing Intestinal Stem Cell Expansion;Jiaqi Zhang, Ruofan Zhang, Yahui Chen, Xin Guo, Yuting Ren, Mengting Wang, Xuan Li, Zan Huang, Weiyun Zhu, Kaifan Yu;2024/08/15;JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
[3]Patient-derived tumor-like cell clusters for personalized chemo- and immunotherapies in non-small cell lung cancer;Shenyi Yin, Ying Yu, Nan Wu, Minglei Zhuo, Yanmin Wang, Yanjie Niu, Yiqian Ni, Fang Hu, Cuiming Ding, Hongsheng Liu, Xinghua Cheng, Jin Peng, Juan Li, Yang He, Jiaxin Li, Junyi Wang, Hanshuo Zhang, Xiaoyu Zhai, Bing Liu, Yaqi;Wang, Shi Yan, Mailin Chen, Wenqing Li, Jincui Peng, Fei Peng, Ruibin Xi, Buqing Ye, Liyan Jiang, Jianzhong Jeff Xi;2024/04/08;Cell Stem Cell
[4]CLMP is a tumor suppressor that determines all-trans retinoic acid response in colorectal cancer;Zhenzhen Wu, Xuanxuan Zhang, Yunhe An, Kaiyue Ma, Ruixin Xue, Gaoqi Ye, Junfeng Du, Zhiyong Chen, Zijing Zhu, Guizhi Shi, Xiang Ding, Meng Wan, Bing Jiang, Peng Zhang, Jinbo Liu, Pengcheng Bu;2023-11-08;DEVELOPMENTAL CELL
[5]Famsin, a novel gut-secreted hormone, contributes to metabolic adaptations to fasting via binding to its receptor OLFR796;Long Aijun, Liu Yang, Fang Xinlei, Jia Liangjie, Li Zhiyuan, Hu Jiang, Wu Shuang, Chen Chao, Huang Ping, Wang Yiguo;2023;CELL RESEARCH
[6]Mesenchymal-epithelial interaction regulates gastrointestinal tract development in mouse embryos;Lianzheng Zhao, Wanlu Song, Ye-Guang Chen;2022;Cell Reports
[7]The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling;Hong Nanchao, Zhang Erge, Xie Huilin, Jin Lihui, Zhang Qi, Lu Yanan, Chen Alex F., Yu Yongguo, Zhou Bin, Chen Sun, Yu Yu, Sun Kun;2021;Cell Death & Disease
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文献支持
Recombinant Mouse Noggin (C-6His)
￥720 - 14400
