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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存:
大量现货
- 供应商:
PeproTech(派普泰克)
- 英文名:
Recombinant Human TGF-beta1-Mammalian
- 规格:
10ug
Human TGF-β1(100-21)
干细胞培养常用因子TGF-β1
转化生长因子β1(TGF-β1)是Tuker等在1984年发现的一种与多种上皮性肿瘤生长有关的多肽性细胞生长负调控因子,是一种同源二聚体,含224个氨基酸残基,分子量约为25千道尔顿(kDa),属于TGF-β超家族,该家族有三种高度同源的TGF-β亚型,即TGF-β1、TGF-β2、TGF-β3。TGF-β1亚型的研究最为广泛。广泛参与体内各种病理生理过程,与炎症、创伤、器官纤维化等多种疾病的发生、发展关系密切,尤其是在肿瘤的发生、发展中的调节作用,对肿瘤的研究具有极其深远的意义。根据癌症基因组图谱(TCGA)的数据,TGF-β1是大多数人类癌症中表达最为普遍的亚型。此外,与TGF-β2和TGF-β3相比,TGF-β1的表达与TGF-β信号激活最密切相关。
来源:HEK293 细胞重组表达
氨基酸序列:ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
蛋白纯度:大于98%
生物活性:The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
内毒素:Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
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我们以Recombinant Human IL-4 (重组 人IL-4,产品编号:200-04)的说明书为例,对细胞因子或蛋白的溶解方法进行详细的阐述。 拿到重组 人IL-4的说明书后,您会发现有一段关于Reconstitution(重悬)的叙述,这段内容含有溶解相关的所有信息。 1. Centrifuge the vial prior to opening 第 1 步:开盖前离心试剂管 PeproTech的细胞因子 或蛋白冻干粉装盛在塑料管中,为无菌包装。冻干
大家知道,PeproTech的所有细胞因子和蛋白均为冻干粉,这使得运输非常便捷,只要常温即可。而且,细胞因子和蛋白冻干粉非常稳定,在-20o C或-80o C条件下可保存数年。冻干粉在使用前需进行溶解,然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键,因溶解不好会导致细胞因子或蛋白的失活,这也是很多用户在实际使用中经常遇到的问题。 应该如何进行正确的溶解呢? 下面我们以Recombinant Human IL-4 (重组人IL-4,产品编号:200-04
Novel Methods for the Investigation of Human Hypertrophic Scarring and Other Dermal Fibrosis
by progressive deposition of collagen that occurs with high frequency in adult dermal wounds following traumatic or thermal injury. Increased levels of transforming growth factor-β1 (TGF-β1 ), decreased expression of small leucine-rich proteoglycans (SLRPs
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