PAK2 Antibody

2608:

Immunoprecipitation , Western Blotting

PAK2 Antibody detects endogenous levels of total PAK2 protein. This antibody does not cross-react with PAK1, PAK3 or PAK4-6.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal region of human PAK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6, COS, guinea pig neutrophils (GPN) and HEK293 cells, using PAK2 Antibody.

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).

1. Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-862.
2. Daniels, R.H. et al. (1998) EMBO J. 17, 754-764.
3. King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-41209.
4. Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-1143.
5. Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8028.
6. Lei, M. et al. (2000) Cell 102, 387-397.
7. Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-17353.
8. Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-3917.
9. Abo, A. et al. (1998) EMBO J. 17, 6527-6540.
10. Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-3533.

• Chu, P. C. et al. (2004) A Novel Role for p21-Activated Protein Kinase 2 in T Cell Activation. The Journal of Immunology 172, 7324-7334. Applications: Western Blotting
• Zhan, Q. et al. (2003) p21-Activated Kinase 2 in Neutrophils Can Be Regulated by Phosphorylation at Multiple Sites and by a Variety of Protein Phosphatases. The Journal of Immunology 171, 3785-3793. Applications: Western Blotting
• Misra, U.K. et al. (2005) J Immunol 175, 2525-33. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 2461 Phospho-Rac1/cdc42 (Ser71) Antibody
• 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
• 2602 PAK1 Antibody
• 2604 PAK1/2/3 Antibody
• 2605 Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
• 2606 Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
• 2607 Phospho-PAK2 (Ser20) Antibody
• 2609 PAK3 Antibody
• 3241 Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody
• 3242 PAK4 Antibody
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• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)

For Research Use Only. Not For Use In Diagnostic Procedures.