mTOR Antibody

2972:

Immunoprecipitation , Western Blotting

mTOR Antibody detects endogenous levels of total mTOR. It cross-reacts weakly with some other proteins based on Western analysis.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower).

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or mTOR siRNA (+). mTOR was detected using mTOR Antibody #2972, and eIF4B was detected using eIF4B Antibody #3592. The mTOR Antibody confirms silencing of mTOR expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
2. Brown, E.J. et al. (1994) Nature 369, 756-8.
3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
6. Fang, Y. et al. (2001) Science 294, 1942-5.
7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

• Suzuki, A. et al. (2004) ARK5 is a tumor invasion-associated factor downstream of Akt signaling. Mol. Cell. Biol. 24, 3526-3535. Applications: Western Blotting
• Takahara, T. et al. (2006) J Biol Chem 281, 28605-14. Applications: Western Blotting
• Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91. Applications: Western Blotting
• Shavlakadze, T. et al. (2010) J Cell Sci , . Applications: Western Blotting
• Chen, C.H. et al. (2011) Sci Signal 4, ra10. Applications: Western Blotting
• Lee, J.W. et al. (2010) PLoS One 5, e15394. Applications: Western Blotting
• Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting
• Gao, D. et al. (2011) Mol Cell 44, 290-303. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 2983 mTOR (7C10) Rabbit mAb
• 4517 mTOR (L27D4) Mouse mAb
• 2971 Phospho-mTOR (Ser2448) Antibody
• 2974 Phospho-mTOR (Ser2481) Antibody
• 2976 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)
• 9904 Rapamycin (FRAP/mTOR Inhibitor)
• 2280 Raptor (24C12) Rabbit mAb
• 3274 GβL (86B8) Rabbit mAb
• 2691 PRAS40 (D23C7) XP^® Rabbit mAb
• 2114 Rictor (53A2) Rabbit mAb
• 9644 4E-BP1 (53H11) Rabbit mAb
• 9205 Phospho-p70 S6 Kinase (Thr389) Antibody
• 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
• 9964 mTOR Pathway Antibody Sampler Kit
• 9862 mTOR Substrates Antibody Sampler Kit
• 9864 mTOR Regulation Antibody Sampler Kit
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.