- 询价
- Cell Signaling Technology
- 4590
- USA
- 2025年08月11日
- IHC-P, IF-F, IF-IC, F
- Rabbit
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Insulin Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human insulin
- 应用范围：
IHC-P, IF-F, IF-IC, F
- 宿主：
Rabbit
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IHC-P IF-F IF-IC F | H M R | Endogenous | 6 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Insulin Antibody detects endogenous levels of total insulin protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β-cells using Insulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β cells, using Insulin Antibody. Flow Cytometry
Flow cytometric analysis of untreated β-TC cells, using Insulin Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of beta-TC-6 and HeLa cells using Insulin Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). IF-F
Confocal immunofluorescent analysis of normal mouse pancreas using Insulin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Detection of Insulin Production by Immunohistochemistry
the insulin gene (4 ). Like all immunocytochemistry techniques, much depends on the availability of a high-quality primary antibody. Species-specific insulin antibodies are now available from a number of commercial sources (including Santa Cruz
Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues
A variety of biochemical and physiological studies in recent years have made it clear that the brain possesses specific receptors and puta- tive signal transduction pathways for insulin and the insulin-like growth factors (IGF-I and IGF
Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest
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