- 询价
- Cell Signaling Technology
- 4941
- USA
- 2025年12月29日
- W, IF-IC, F
- Rabbit
- H,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-PBK/TOPK (Thr9) Antibody
- 抗原：
synthetic phosphopeptide corresponding to amino acids around Thr9 of human PBK/TOPK
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H M | Endogenous | 40 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-PBK/TOPK (Thr9) Antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to amino acids around Thr9 of human PBK/TOPK. Antibodies are purified by protein A and affinity chromatography. Western Blotting
Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-PBK/TOPK (Thr9) Antibody (upper) or PBK/TOPK Antibody #4942 (lower). Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells, using Phospho-PBK/TOPK (Thr9) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-PBK/TOPK (Thr9)-positive cells. IF-IC
Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-PBK/TOPK (Thr9) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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