IκBα Antibody

9242:

Immunoprecipitation , Western Blotting

IκBα Antibody detects endogenous levels of total IκBα protein. Under some experimental conditions it binds preferentially to IκBα that is not phosphorylated at Ser32/Ser36.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Arg29 of human IκBα. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa cells treated with TNF-α (#2169, 20 ng/ml) for the indicated times, usingIκBα Antibody.

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

• Bonnard, M. et al. (2000) Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-kappaB-dependent gene transcription. EMBO J. 19, 4976-4985. Applications: Western Blotting
• Burns, K. et al. (2000) Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nat. Cell Biol. 2, 346-351. Applications: Western Blotting
• Delehedde, M. et al. (2000) Fibroblast growth factor-2 stimulation of p42/44MAPK phosphorylation and ikappa B degradation is regulated by heparan sulfate/heparin in rat mammary fibroblasts. J. Biol. Chem. 275, 33905-33910. Applications: Western Blotting
• Hoeflich, K. P. et al. (2000) Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation. Nature 406, 86-90. Applications: Western Blotting
• Todd, D. E. et al. (2004) ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest. Oncogene 23, 3284-3295. Applications: Western Blotting
• Casciati, A. et al. (2002) Oxidative modulation of nuclear factor-kappaB in human cells expressing mutant fALS-typical superoxide dismutases. J Neurochem 83, 1019-1029. Applications: Western Blotting

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• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.