Applications Key: W=Western Blotting Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
SKARα/β Antibody detects endogenous levels of total SKAR protein. The antibody recognizes both the α and the β isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino terminal residues of human SKAR. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using SKARα/β Antibody.
Background
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3).