Connexin 43 Antibody

价格询价

品牌Cell Signaling Technology
货号
3512
产地USA
更新日期2026年05月28日

应用范围W, IHC-P, IHC-F, IF-F, IF-IC
宿主Rabbit
适应物种H,M,R,Mk,Z,Dg,Pg

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
询价记录
文献和实验
技术资料

抗体英文名：
Connexin 43 Antibody
抗原：
synthetic peptide corresponding to residues of human connexin 43
应用范围：
W, IHC-P, IHC-F, IF-F, IF-IC
宿主：
Rabbit
供应商：
CST
级别：
详见MSDS文件
库存：
大量
保质期：
详见说明书
适应物种：
H,M,R,Mk,Z,Dg,Pg
是否单克隆：
2
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IHC-F IF-F IF-IC	H M R Mk Z (Dg) (Pg)	Endogenous	39, 41, 43, 44	Rabbit

Protocols	3512: IHC / Frozen , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity	Connexin 43 Antibody detects endogenous levels of total connexin 43. This antibody does not cross-react with other connexins.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human connexin 43. Antibodies are purified by protein A and peptide affinity chromatography Western Blotting Western blot analysis of extracts from untreated and PMA-treated C6, COS, HeLa and C2C12 cells using Connexin 43 Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Connexin 43 Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human heart, using Connexin 43 Antibody. (High magnification, inset). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Connexin 43 Antibody. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded mouse heart using Connexin 43 Antibody in the presence of control peptide (left) or antigen specific peptide (lright). IHC-F (frozen) Immunohistochemical analysis of frozen mouse heart tissue, using Connexin 43 Antibody. IF-IC Confocal immunofluorescent analysis of confluent COS cells using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). IF-F Confocal immunofluorescent analysis of rat optic nerve and ciliary epithelium using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).See notes for additional testing details.
Background	Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5). Musil, L. S. et al. (1990) J. Cell. Biol. 111, 2077-2088. Musil, L.S. and Goodenough, D.A. (1991) J. Cell. Biol. 115, 1357-1374. Lampe, P. D. et al. (2000) J. Cell. Biol. 149, 1503-1512. Giepmans, B. N. et al. (2001) J. Biol. Chem. 276, 8544-8549. Lin, R. et al. (2001) J. Cell. Biol. 154, 815-827.
Application References	Zhang, Q. et al. (2006) Am J Physiol Regul Integr Comp Physiol 291, R1688-99. Applications: IF-IC (In Cells) Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	3511 Phospho-Connexin 43 (Ser368) Antibody 9371 Phospho-PKC (pan) (βII Ser660) Antibody 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 8112 SignalStain^® Antibody Diluent 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) For Research Use Only. Not For Use In Diagnostic Procedures.