- 询价
- Cell Signaling Technology
- 3310
- USA
- 2025年07月15日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 级别：
详见MSDS文件
- 适应物种：
人
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 抗体英文名：
c-Kit (Ab81) Mouse mAb (Alexa Fluor 488 Conjugate)
- 是否单克隆：
1
- 库存：
大量
- 宿主：
小鼠
- 供应商：
CST
- 抗原：
/
- 抗原来源：
/
- 保质期：
详见说明书
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Tyrosine Kinase / Adaptors
c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate) #3310
PhosphoSitePlus ® protein, site, and accession data: Kit
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H | Endogenous | Mouse IgG1 |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3310:
- Flow , Immunofluorescence
Specificity / Sensitivity
c-Kit (Ab81) Mouse mAb detects endogenous levels of total c-Kit protein. It does not cross-react with other receptor tyrosine kinase family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant proteins containing the cytoplasmic domain of human c-Kit. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat (blue) and H526 (green) cells using c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate).
IF-IC
Confocal immunofluorescent analysis of H526 cells using c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells.
Background
c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
- Martin, F.H. et al. (1990) Cell 63, 203-11.
- Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
- Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
- Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
- Nocka, K. et al. (1990) EMBO J 9, 1805-13.
- Hirota, S. et al. (1998) Science 279, 577-80.
- Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 3073 Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb
- 3074 c-Kit (D13A2) XP® Rabbit mAb
- 3308 c-Kit (Ab81) Mouse mAb
- 3391 Phospho-c-Kit (Tyr719) Antibody
- 3392 c-Kit Antibody
- 4878 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验：Catalog NumberProduct NameProduct TypeSizeApplication4408SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F HCA4409SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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