Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor(R) 488 Conjugate)产品图

Phospho-p38 MAPK (Thr180/Tyr18

2) (28B10) Mouse mAb (Alexa Fluor(R) 488 Conjugate)
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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2026年08月20日
  • 流式细胞（Flow Cyt）
  • 小鼠
  • 人,小鼠,大鼠,驴,酵母菌,斑马鱼
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商：

      CST

    • 抗原：

      /

    • 宿主：

      小鼠

    • 保存条件：

      -20°c

    • 抗体英文名：

      Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor 488 Conjugate)

    • 是否单克隆：

      1

    • 库存：

      大量

    • 适应物种：

      人,小鼠,大鼠,驴,酵母菌,斑马鱼

    • 应用范围：

      流式细胞（Flow Cyt）

    • 抗原来源：

      /

    • 保质期：

      详见说明书

    • 级别：

      详见MSDS文件

    • 规格：

      100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a>

    规格：产品价格：￥面议
    规格：100 ul (50 tests)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价
    规格：100 ul (50 tests)产品价格：￥请询价
    规格：<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free &amp; custom formulation / quantity</a> 产品价格：￥请询价

    Product Pathways - MAPK Signaling

    Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) #4551

    Applications Reactivity Sensitivity MW (kDa) Isotype
    F H M R Mk Sc (Z) Endogenous 40 Mouse IgG1

    Applications Key:  F=Flow Cytometry
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Z=Zebrafish  Sc=S. cerevisiae
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Protocols

    Specificity / Sensitivity

    Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not appreciably cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase. Antibody was conjugated to Alexa Fluor® 488 under optimum conditions with an F/P ratio of 2-6.

    Flow Cytometry

    Flow Cytometry

    Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) compared to a nonspecific control antibody (red).

    Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human and mouse cells. The unconjugated antibody #9216 reacts with human, mouse, rat, D. melanogaster, monkey, S. cerevisiae and zebra fish phospho-p38 MAPK (Thr180/Tyr182). CST expects that Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 488 Conjugate) will also recognize p44/42 MAPK in these species.

    Background

    p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

    1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
    2. Han, J. et al. (1994) Science 265, 808-811.
    3. Lee, J.C. et al. (1994) Nature 372, 739-746.
    4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
    5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
    6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
    7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
    8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
    9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
    10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

    For Research Use Only. Not For Use In Diagnostic Procedures.

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