GSK-3β (27C10) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
9315
产地USA
更新日期2026年05月28日

应用范围W, IP, IHC-P
适应物种H,M,R,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
GSK-3β (27C10) Rabbit mAb
抗原：
synthetic peptide corresponding to the sequence of human GSK-3β
应用范围：
W, IP, IHC-P
供应商：
CST
适应物种：
H,M,R,Mk
库存：
大量
级别：
详见MSDS文件
保质期：
详见说明书
是否单克隆：
1
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	300 ul (30 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P	H M R Mk	Endogenous	46	Rabbit IgG

Protocols	9315: IHC / Paraffin , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	GSK-3β (27C10) Rabbit mAb detects endogenous levels of total GSK-3β protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GSK-3β. Western Blotting Western blot analysis of extracts from HeLa, NIH/3T3, COS, C6 and 293 cells using GSK-3β (27C10) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization using GSK-3β (27C10) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung carcinoma using GSK-3β (27C10) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using GSK-3beta (27C10) Rabbit mAb in the presence of control peptide (left) or GSK-3Beta Blocking Peptide #1073 (right).
Background	Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141. Cross, D.A. et al. (1995) Nature 378, 785-789. Nusse, R. (1997) Cell 89, 321-323. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.
Application References	Laguë, M.N. et al. (2008) Carcinogenesis 29, 2062-72. Applications: Western Blotting Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. Applications: Western Blotting Wu, M. et al. (2009) PLoS One 4, e6430. Applications: Western Blotting Wang, J. et al. (2009) J Histochem Cytochem 57, 363-71. Applications: IHC-P (paraffin) Perez-Costas, E. et al. (2010) PLoS One 5, e8911. Applications: Immunological Electron Microscopy Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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