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- Corning
- 354236
- 美国
- 2025年12月31日
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- 库存：
9999
- 规格：
100mg
Corning® Collagen I, rat tail, 100mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function.
Source
Rat Tail Tendon
Size
100mg
Quality
Purity = 90% by SDS-PAGE
Tested for: ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells
Forms firm gel up to a dilution of 1:10; further dilution will decrease rigidity of gel
Tested and found negative for bacteria, fungi, and mycoplasma
Formulation
Liquid in 0.02 N acetic acid, concentration range 3-4mg/ mL
Preparation and Storage
Stable for at least three months at 2° to 8°C. Do not freeze.
Handling
Effective as a gel or thin coating at a recommended concentration of 5-10µg/cm² of growth surface depending on cell type
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文献和实验Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)
75?) 1.5mL 10x reconstitution buffer 1.5mL 10x DMEM 12mL rat tail type 1 collagen (>3.8mg/mL) 10N NaOH Glacial acetic acid (in case) Method Pre-chill pipettes
Dorsal Root Ganglion Neuron Culture from Adult Rats
Reagents - Euthanasia Drugs (10:3 Ketamine-Rompun or Phenobarbital) - Rat-tail collagen - Calf serum - 2.5% collagenase - 0.5% trypsin - Neuralbasal-A media (Gibco) - B-27 with antioxidants - L-glutamine - Pen-strep-neo - OPTIONAL: NGF
Dorsal Root Ganglion Neuron Culture from Adult Rats
Reagents - Euthanasia Drugs (10:3 Ketamine-Rompun or Phenobarbital) - Rat-tail collagen - Calf serum - 2.5% collagenase - 0.5% trypsin - Neuralbasal-A media (Gibco) - B-27 with antioxidants - L-glutamine - Pen-strep-neo - OPTIONAL: NGF
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