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文献和实验鼠尾制备胶原蛋白-Making collagen solution from lyophilized rat tail collagen
Making collagen solution from lyophilized rat tail collagen鼠尾制备胶原蛋白 Chen-Ming Fan Lab,Carnegie Institute of Washington (lyophilized rat tail collagen from Roche) DME power from Life technologies (buy the one without NaHCO
primary fibroblasts (˜ cell number 1 x 106 ) use: A. 25 ml rat tail collagen (approx conc 2mg/ml) B. 3 ml 10xMEM C. 0.22 M NaOH: first, add 2ml, then drop-wise whilst stirring, until the collagen turns
Preparing Mitochondria from Rat Liver
medium to tissue is important, as is the depth of liquid in the container when using a shearing type homogenizer. The specific instrument we use is a "Tissuemizer" with T25 stainless steel shaft (Tekmar, Inc., Cincinnatti, OH). A teflon-on-glass (Potter
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